Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation

Tadafumi Asaoka; Tomoaki Kato; Shigeru Marubashi; Keizo Dono; Naoki Hama; Hidenori Takahashi; Shogo Kobayashi; Yutaka Takeda; Ichiro Takemasa; Hiroaki Nagano; Hideo Yoshida; Phillip Ruiz; Andreas G. Tzakis; Kenichi Matsubara; Morito Monden; Yuichiro Doki; Masaki Mori

doi:10.1002/lt.21883

Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation

Tadafumi Asaoka, Tomoaki Kato, Shigeru Marubashi, Keizo Dono, Naoki Hama, Hidenori Takahashi, Shogo Kobayashi, Yutaka Takeda, Ichiro Takemasa, Hiroaki Nagano, Hideo Yoshida, Phillip Ruiz, Andreas G. Tzakis, Kenichi Matsubara, Morito Monden, Yuichiro Doki, Masaki Mori

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Histopathological evaluation of the liver via biopsy remains the standard procedure for the diagnosis of both acute cellular rejection (ACR) and recurrent hepatitis C (RHC) after liver transplantation. Nevertheless, it is often difficult to diagnose ACR in hepatitis C virus-positive recipients because of changes in common and overlapping with RHC. The aim of this study was to identify potential target genes for ACR in recipients with RHC. We analyzed 22 liver biopsy samples obtained from 21 hepatitis C virus-positive recipients. The clinicopathological diagnosis based on biopsy examination was ACR-predominant with superimposed RHC in 9 samples (ACR group) and RHC without ACR (non-ACR group) in 13. Using oligonucleotide microarrays, we compared the transcriptional changes in the 2 groups and selected 2206 genes that were significantly modulated in ACR. We analyzed the regulatory networks in ACR with Ingenuity Pathway Analysis software, and we confirmed with quantitative real-time polymerase chain reaction the reproducibility of caspase 8, apoptosis-related cysteine peptidase and bone morphogenetic protein 2 up-regulation in another group of validation samples, representing 2 genes from the core network as the target genes for ACR. Our results demonstrated novel transcriptome patterns for ACR with concurrent RHC that were distinct from those of recipients with only RHC, suggesting that gene expression profiling may be useful in the diagnosis of ACR in recipients with hepatitis C.

Original language	English
Pages (from-to)	1738-1749
Number of pages	12
Journal	Liver Transplantation
Volume	15
Issue number	12
DOIs	https://doi.org/10.1002/lt.21883
Publication status	Published - Dec 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Surgery
Hepatology
Transplantation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/lt.21883

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Asaoka, T., Kato, T., Marubashi, S., Dono, K., Hama, N., Takahashi, H., Kobayashi, S., Takeda, Y., Takemasa, I., Nagano, H., Yoshida, H., Ruiz, P., Tzakis, A. G., Matsubara, K., Monden, M., Doki, Y., & Mori, M. (2009). Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation. Liver Transplantation, 15(12), 1738-1749. https://doi.org/10.1002/lt.21883

Asaoka, T, Kato, T, Marubashi, S, Dono, K, Hama, N, Takahashi, H, Kobayashi, S, Takeda, Y, Takemasa, I, Nagano, H, Yoshida, H, Ruiz, P, Tzakis, AG, Matsubara, K, Monden, M, Doki, Y & Mori, M 2009, 'Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation', Liver Transplantation, vol. 15, no. 12, pp. 1738-1749. https://doi.org/10.1002/lt.21883

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abstract = "Histopathological evaluation of the liver via biopsy remains the standard procedure for the diagnosis of both acute cellular rejection (ACR) and recurrent hepatitis C (RHC) after liver transplantation. Nevertheless, it is often difficult to diagnose ACR in hepatitis C virus-positive recipients because of changes in common and overlapping with RHC. The aim of this study was to identify potential target genes for ACR in recipients with RHC. We analyzed 22 liver biopsy samples obtained from 21 hepatitis C virus-positive recipients. The clinicopathological diagnosis based on biopsy examination was ACR-predominant with superimposed RHC in 9 samples (ACR group) and RHC without ACR (non-ACR group) in 13. Using oligonucleotide microarrays, we compared the transcriptional changes in the 2 groups and selected 2206 genes that were significantly modulated in ACR. We analyzed the regulatory networks in ACR with Ingenuity Pathway Analysis software, and we confirmed with quantitative real-time polymerase chain reaction the reproducibility of caspase 8, apoptosis-related cysteine peptidase and bone morphogenetic protein 2 up-regulation in another group of validation samples, representing 2 genes from the core network as the target genes for ACR. Our results demonstrated novel transcriptome patterns for ACR with concurrent RHC that were distinct from those of recipients with only RHC, suggesting that gene expression profiling may be useful in the diagnosis of ACR in recipients with hepatitis C.",

author = "Tadafumi Asaoka and Tomoaki Kato and Shigeru Marubashi and Keizo Dono and Naoki Hama and Hidenori Takahashi and Shogo Kobayashi and Yutaka Takeda and Ichiro Takemasa and Hiroaki Nagano and Hideo Yoshida and Phillip Ruiz and Tzakis, {Andreas G.} and Kenichi Matsubara and Morito Monden and Yuichiro Doki and Masaki Mori",

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AU - Asaoka, Tadafumi

AU - Kato, Tomoaki

AU - Marubashi, Shigeru

AU - Dono, Keizo

AU - Hama, Naoki

AU - Takahashi, Hidenori

AU - Kobayashi, Shogo

AU - Takeda, Yutaka

AU - Takemasa, Ichiro

AU - Nagano, Hiroaki

AU - Yoshida, Hideo

AU - Ruiz, Phillip

AU - Tzakis, Andreas G.

AU - Matsubara, Kenichi

AU - Monden, Morito

AU - Doki, Yuichiro

AU - Mori, Masaki

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AB - Histopathological evaluation of the liver via biopsy remains the standard procedure for the diagnosis of both acute cellular rejection (ACR) and recurrent hepatitis C (RHC) after liver transplantation. Nevertheless, it is often difficult to diagnose ACR in hepatitis C virus-positive recipients because of changes in common and overlapping with RHC. The aim of this study was to identify potential target genes for ACR in recipients with RHC. We analyzed 22 liver biopsy samples obtained from 21 hepatitis C virus-positive recipients. The clinicopathological diagnosis based on biopsy examination was ACR-predominant with superimposed RHC in 9 samples (ACR group) and RHC without ACR (non-ACR group) in 13. Using oligonucleotide microarrays, we compared the transcriptional changes in the 2 groups and selected 2206 genes that were significantly modulated in ACR. We analyzed the regulatory networks in ACR with Ingenuity Pathway Analysis software, and we confirmed with quantitative real-time polymerase chain reaction the reproducibility of caspase 8, apoptosis-related cysteine peptidase and bone morphogenetic protein 2 up-regulation in another group of validation samples, representing 2 genes from the core network as the target genes for ACR. Our results demonstrated novel transcriptome patterns for ACR with concurrent RHC that were distinct from those of recipients with only RHC, suggesting that gene expression profiling may be useful in the diagnosis of ACR in recipients with hepatitis C.

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Differential transcriptome patterns for acute cellular rejection in recipients with recurrent hepatitis C after liver transplantation

Abstract

All Science Journal Classification (ASJC) codes

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