Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Yutaka Okuno; Hiromi Iwasaki; Claudia S. Huettner; Hanna S. Radomska; David A. Gonzalez; Daniel G. Tenen; Koichi Akashi

doi:10.1073/pnas.092027799

Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Yutaka Okuno, Hiromi Iwasaki, Claudia S. Huettner, Hanna S. Radomska, David A. Gonzalez, Daniel G. Tenen, Koichi Akashi

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34^-/lo express the hCD34 transgene. These data strongly support the notion that hCD34⁺ human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

Original language	English
Pages (from-to)	6246-6251
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	9
DOIs	https://doi.org/10.1073/pnas.092027799
Publication status	Published - Apr 30 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

General

Access to Document

10.1073/pnas.092027799

Cite this

@article{60d1f24be40a4053b3a7ab05efb73361,

title = "Differential regulation of the human and murine CD34 genes in hematopoietic stem cells",

abstract = "Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34-/lo express the hCD34 transgene. These data strongly support the notion that hCD34+ human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.",

author = "Yutaka Okuno and Hiromi Iwasaki and Huettner, {Claudia S.} and Radomska, {Hanna S.} and Gonzalez, {David A.} and Tenen, {Daniel G.} and Koichi Akashi",

year = "2002",

month = apr,

day = "30",

doi = "10.1073/pnas.092027799",

language = "English",

volume = "99",

pages = "6246--6251",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "9",

}

TY - JOUR

T1 - Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

AU - Okuno, Yutaka

AU - Iwasaki, Hiromi

AU - Huettner, Claudia S.

AU - Radomska, Hanna S.

AU - Gonzalez, David A.

AU - Tenen, Daniel G.

AU - Akashi, Koichi

PY - 2002/4/30

Y1 - 2002/4/30

N2 - Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34-/lo express the hCD34 transgene. These data strongly support the notion that hCD34+ human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

AB - Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic P1 artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34-/lo express the hCD34 transgene. These data strongly support the notion that hCD34+ human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

UR - http://www.scopus.com/inward/record.url?scp=0037197968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037197968&partnerID=8YFLogxK

U2 - 10.1073/pnas.092027799

DO - 10.1073/pnas.092027799

M3 - Article

C2 - 11983914

AN - SCOPUS:0037197968

SN - 0027-8424

VL - 99

SP - 6246

EP - 6251

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -

Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this