TY - JOUR
T1 - Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast
AU - Miura, Fumihito
AU - Yada, Tetsushi
AU - Nakai, Kenta
AU - Sakaki, Yoshiyuki
AU - Ito, Takashi
N1 - Funding Information:
We thank Andre Goffeau for yeast strains and encouragement. This work was supported by research grants from Japan Society for the Promotion of Sciences (JSPS) and the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
PY - 2001/9/7
Y1 - 2001/9/7
N2 - The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well-known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.
AB - The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well-known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.
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U2 - 10.1016/S0014-5793(01)02792-2
DO - 10.1016/S0014-5793(01)02792-2
M3 - Article
C2 - 11557050
AN - SCOPUS:0035823109
SN - 0014-5793
VL - 505
SP - 103
EP - 108
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -