TY - JOUR
T1 - Differences between strains in the tolerance to testis cryopreservation in the silkworm, Bombyx mori
AU - Fukumori, Hisayoshi
AU - Fujii, Tsuguru
AU - Tanaka, Daisuke
AU - Banno, Yutaka
N1 - Funding Information:
Funding: This work was supported by the Ministry of
Funding Information:
The use of silkworm strains in this study was supported by the National BioResource Project (NBRP) of the MEXT, Japan. This work was supported by the NIBB Collaborative Research Program (21-605) to YB. We would like to thank Enago (www.enago.jp) for the English language review.
Funding Information:
The use of silkworm strains in this study was supported by the National BioResource Project (NBRP) of the MEXT, Japan. This work was supported by the NIBB Collaborative Research Program (21-605) to YB. We would like to thank Enago (www.enago.jp) for the English language review. This work was supported by the Ministry of Education, Science, Sports and Culture of Japan, National BioResource Project (NBRP). The work presented here was supported in part as a Collaborative Research Project organized by the Interuniversity Bio-Backup Project (IBBP) of National Institute for Basic Biology.
Publisher Copyright:
© 2022, Japanese Society of Sericultural Sciences. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Long-term cryopreservation of the Bombyx mori silkworm is currently performed using ovaries and mature sperm. These methods have been in practice in the cryopreservation of many silkworm strains. Development of multiple methods for the safe preservation of bioresources is desirable. In this study, we investigated the potential for silkworm testis cryopreservation. We show here that the viability of cryopreservation using testis varied among the strains. For viable long-term testis cryopreservation, increasing the success rate for low fertility strains is essential. We investigated a vitrification method for testis using aluminum cryo-plates. There was the possibility, however, more experiments are required to improve this vitrification method for silkworm testis cryopreservation.
AB - Long-term cryopreservation of the Bombyx mori silkworm is currently performed using ovaries and mature sperm. These methods have been in practice in the cryopreservation of many silkworm strains. Development of multiple methods for the safe preservation of bioresources is desirable. In this study, we investigated the potential for silkworm testis cryopreservation. We show here that the viability of cryopreservation using testis varied among the strains. For viable long-term testis cryopreservation, increasing the success rate for low fertility strains is essential. We investigated a vitrification method for testis using aluminum cryo-plates. There was the possibility, however, more experiments are required to improve this vitrification method for silkworm testis cryopreservation.
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U2 - 10.11416/jibs.91.1_013
DO - 10.11416/jibs.91.1_013
M3 - Article
AN - SCOPUS:85134215176
SN - 1346-8073
VL - 91
SP - 13
EP - 19
JO - Journal of Insect Biotechnology and Sericology
JF - Journal of Insect Biotechnology and Sericology
IS - 1
ER -