TY - JOUR
T1 - Dielectrophoretic filter for separation and recovery of biological cells in water
AU - Suehiro, Junya
AU - Zhou, Guangbin
AU - Imamura, Manabu
AU - Hara, Masanori
N1 - Funding Information:
Paper MSDAD-A 03–06, presented at the 2002 Industry Applications Society Annual Meeting, Pittsburgh, PA, October 13–18, and approved for publication in the IEEE TRANSACTIONS ON INDUSTRY APPLICATIONS by the Electrostatic Processes Committee of the IEEE Industry Applications Society. Manuscript submitted for review October 15, 2002 and released for publication May 27, 2003. This work was supported in part by a research fund from the Mukai Science and Technology Foundation, Japan, and in part by Grant-in-Aid for Scientific Research 14550421 from the Japan Society for the Promotion of Science.
PY - 2003/9
Y1 - 2003/9
N2 - Dielectrophoresis (DEP) is the electrokinetic motion of dielectrically polarized particles in nonuniform electric fields. DEP has found many useful technological applications including separation, levitation, and characterization of dielectric particles such as biological cells. In this study, the authors demonstrated continuous separation and recovery of biological cells suspended in water using a DEP filter. The DEP filter consists of an electrode system that is filled up with many glass beads. These glass beads modify the electric field distribution in the electrode system so that strong DEP force is generated on their surfaces. If the DEP force is stronger than drag force exerted by liquid flow in the filter, the suspended particles can be trapped and eliminated from the flowing liquid. The DEP filter can control trapping and releasing process just by changing electrode energizing ac signal and the resultant DEP force. It was experimentally confirmed that the DEP filter could continuously eliminate yeast cells suspended in water. The cell density decreased from 106 to 101 cells/mL in about 1 h. The electrical conductivity of the medium was a crucial parameter that influenced the liquid temperature by Joule heating and DEP force. Furthermore, the selective separation of viable and nonviable yeast cells was demonstrated by utilizing the viability dependency of the DEP force.
AB - Dielectrophoresis (DEP) is the electrokinetic motion of dielectrically polarized particles in nonuniform electric fields. DEP has found many useful technological applications including separation, levitation, and characterization of dielectric particles such as biological cells. In this study, the authors demonstrated continuous separation and recovery of biological cells suspended in water using a DEP filter. The DEP filter consists of an electrode system that is filled up with many glass beads. These glass beads modify the electric field distribution in the electrode system so that strong DEP force is generated on their surfaces. If the DEP force is stronger than drag force exerted by liquid flow in the filter, the suspended particles can be trapped and eliminated from the flowing liquid. The DEP filter can control trapping and releasing process just by changing electrode energizing ac signal and the resultant DEP force. It was experimentally confirmed that the DEP filter could continuously eliminate yeast cells suspended in water. The cell density decreased from 106 to 101 cells/mL in about 1 h. The electrical conductivity of the medium was a crucial parameter that influenced the liquid temperature by Joule heating and DEP force. Furthermore, the selective separation of viable and nonviable yeast cells was demonstrated by utilizing the viability dependency of the DEP force.
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U2 - 10.1109/TIA.2003.816535
DO - 10.1109/TIA.2003.816535
M3 - Article
AN - SCOPUS:0242368957
SN - 0093-9994
VL - 39
SP - 1514
EP - 1521
JO - IEEE Transactions on Industry Applications
JF - IEEE Transactions on Industry Applications
IS - 5
ER -