TY - JOUR
T1 - Diadema setosum
T2 - isolation of bioactive secondary metabolites with cytotoxic activity toward human cervical cancer
AU - Abdelkarem, Fahd M.
AU - Desoky, Ezz Eldin K.
AU - Nafady, Alaa M.
AU - Allam, Ahmed E.
AU - Mahdy, Aldoushy
AU - Ashour, Ahmed
AU - Shimizu, Kuniyoshi
N1 - Funding Information:
Gratefully, Abdelkarem FM acknowledge the Ministry of Higher Education, Egypt for the scholarship support.
Publisher Copyright:
© 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022
Y1 - 2022
N2 - In our promising project toward discovery of secondary metabolites with potential anticancer activity against human cervical cancer, seven marine organisms were screened for their cytotoxic activity against HeLa cancer cell line using MTT colorimetric assay. The crude extract of the outer shell of Diadema setosum showed promising activity with 88.02% inhibition at a concentration 250 µg/ml. Chromatographic investigation of the Ethyl acetate fraction, which is the main contributor to the activity (IC50= 43.1 ± 5.94 µg/ml), led to isolation of five compounds. Structures of the isolates (1-5) were elucidated by 1 D and 2 D NMR spectroscopy and HR-ESI-MS analysis. 5α,8α-epidioxycholest-6-en-3β ol (2) and 5α,8α-epidioxycholest-6,9(11)-en-3β ol (3) showed the highest cytotoxic activity with IC50 values 12.1 ± 2.74 µg/ml and 21.8 ± 6.32 µg/ml, respectively. Epidioxy steroids with cholestane nucleus could be a prospective candidate for the development of drugs for treatment of human cervical cancer.
AB - In our promising project toward discovery of secondary metabolites with potential anticancer activity against human cervical cancer, seven marine organisms were screened for their cytotoxic activity against HeLa cancer cell line using MTT colorimetric assay. The crude extract of the outer shell of Diadema setosum showed promising activity with 88.02% inhibition at a concentration 250 µg/ml. Chromatographic investigation of the Ethyl acetate fraction, which is the main contributor to the activity (IC50= 43.1 ± 5.94 µg/ml), led to isolation of five compounds. Structures of the isolates (1-5) were elucidated by 1 D and 2 D NMR spectroscopy and HR-ESI-MS analysis. 5α,8α-epidioxycholest-6-en-3β ol (2) and 5α,8α-epidioxycholest-6,9(11)-en-3β ol (3) showed the highest cytotoxic activity with IC50 values 12.1 ± 2.74 µg/ml and 21.8 ± 6.32 µg/ml, respectively. Epidioxy steroids with cholestane nucleus could be a prospective candidate for the development of drugs for treatment of human cervical cancer.
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U2 - 10.1080/14786419.2020.1855162
DO - 10.1080/14786419.2020.1855162
M3 - Article
C2 - 33274647
AN - SCOPUS:85097075348
SN - 1478-6419
VL - 36
SP - 1118
EP - 1122
JO - Natural Product Research
JF - Natural Product Research
IS - 4
ER -
