Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro

Naoto Burioka; Miyako Takata; Yoko Okano; Shigehiro Ohdo; Yasushi Fukuoka; Masanori Miyata; Hiroshi Takane; Masahiro Endo; Hisashi Suyama; Eiji Shimizu

doi:10.1081/CBI-200062416

Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro

Naoto Burioka, Miyako Takata, Yoko Okano, Shigehiro Ohdo, Yasushi Fukuoka, Masanori Miyata, Hiroshi Takane, Masahiro Endo, Hisashi Suyama, Eiji Shimizu

Pharmaceutical Health Care and Sciences Faculty

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.

Original language	English
Pages (from-to)	585-590
Number of pages	6
Journal	Chronobiology International
Volume	22
Issue number	3
DOIs	https://doi.org/10.1081/CBI-200062416
Publication status	Published - 2005

All Science Journal Classification (ASJC) codes

Physiology
Physiology (medical)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1081/CBI-200062416

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title = "Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro",

abstract = "We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.",

author = "Naoto Burioka and Miyako Takata and Yoko Okano and Shigehiro Ohdo and Yasushi Fukuoka and Masanori Miyata and Hiroshi Takane and Masahiro Endo and Hisashi Suyama and Eiji Shimizu",

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T1 - Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro

AU - Burioka, Naoto

AU - Takata, Miyako

AU - Okano, Yoko

AU - Ohdo, Shigehiro

AU - Fukuoka, Yasushi

AU - Miyata, Masanori

AU - Takane, Hiroshi

AU - Endo, Masahiro

AU - Suyama, Hisashi

AU - Shimizu, Eiji

PY - 2005

Y1 - 2005

N2 - We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.

AB - We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (∼07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.

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EP - 590

JO - Chronobiology International

JF - Chronobiology International

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ER -

Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro

Abstract

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