Development of surface plasmon resonance imaging apparatus for high-throughput study of protein-surface interactions

We report a parallel analysis of protein-surface interactions using an array presenting spots with different surface properties and a surface plasmon resonance (SPR) imaging apparatus. The array was fabricated by the photopatterning of self-assembled monolayers (SAMs) carrying different functional groups including methyl, hydroxyl, carboxylic acid, amine, and poly(ethylene glycol)-tethered amine. The SPR imaging apparatus was developed in-house. The adsorption behavior of bovine serum albumin (BSA) on SAMs was monitored by SPR imaging. The adsorption amounts obtained using the SPR imaging apparatus were well correlated with those obtained by the bicinchoninic acid (BCA) assay. The combination of the array and SPR imaging is suitable for high-throughput, real-time, label-free, and highly-sensitive studies of the interactions of proteins with surfaces with various properties.