Development of rapid in vitro assay for oxidative liver injury and its application to 230 chemicals

S. H. Kim; K. Ichikawa; I. Koshiishi; H. Utsumi

doi:10.2166/wst.2002.0759

Development of rapid in vitro assay for oxidative liver injury and its application to 230 chemicals

S. H. Kim, K. Ichikawa, I. Koshiishi, H. Utsumi

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Water environments are thought to be polluted with thousans of synthetic chemicals and biproducts involving persistent organic pollutants and endocrine disrupters, and their human and ecological impacts are causing serious anxiety. Many bioassays have been undertaken to evaluate the hazardous impacts of toxic chemicals dissolved in water. Reactive Oxygen Species (ROS) are well known to be involved in the toxicity of various chemicals. ROS are mostly generated in liver and cause oxidative damage to DNA, lipids and proteins, resulting in the failure of cellular functions. In order to develop an in vitro bioassay system to estimate ROS induced liver toxicity by chemicals, we investigated the lipid peroxidation, liver cell injury, and genotoxicity using the human hepatocellular carcinoma cell line, Hep-G2 (ATCC HB 8065), and applied it to 230 chemicals.

Original language	English
Pages (from-to)	337-341
Number of pages	5
Journal	Water Science and Technology
Volume	46
Issue number	11-12
DOIs	https://doi.org/10.2166/wst.2002.0759
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Environmental Engineering
Water Science and Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2166/wst.2002.0759

Cite this

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title = "Development of rapid in vitro assay for oxidative liver injury and its application to 230 chemicals",

abstract = "Water environments are thought to be polluted with thousans of synthetic chemicals and biproducts involving persistent organic pollutants and endocrine disrupters, and their human and ecological impacts are causing serious anxiety. Many bioassays have been undertaken to evaluate the hazardous impacts of toxic chemicals dissolved in water. Reactive Oxygen Species (ROS) are well known to be involved in the toxicity of various chemicals. ROS are mostly generated in liver and cause oxidative damage to DNA, lipids and proteins, resulting in the failure of cellular functions. In order to develop an in vitro bioassay system to estimate ROS induced liver toxicity by chemicals, we investigated the lipid peroxidation, liver cell injury, and genotoxicity using the human hepatocellular carcinoma cell line, Hep-G2 (ATCC HB 8065), and applied it to 230 chemicals.",

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T1 - Development of rapid in vitro assay for oxidative liver injury and its application to 230 chemicals

AU - Kim, S. H.

AU - Ichikawa, K.

AU - Koshiishi, I.

AU - Utsumi, H.

PY - 2002

Y1 - 2002

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AB - Water environments are thought to be polluted with thousans of synthetic chemicals and biproducts involving persistent organic pollutants and endocrine disrupters, and their human and ecological impacts are causing serious anxiety. Many bioassays have been undertaken to evaluate the hazardous impacts of toxic chemicals dissolved in water. Reactive Oxygen Species (ROS) are well known to be involved in the toxicity of various chemicals. ROS are mostly generated in liver and cause oxidative damage to DNA, lipids and proteins, resulting in the failure of cellular functions. In order to develop an in vitro bioassay system to estimate ROS induced liver toxicity by chemicals, we investigated the lipid peroxidation, liver cell injury, and genotoxicity using the human hepatocellular carcinoma cell line, Hep-G2 (ATCC HB 8065), and applied it to 230 chemicals.

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Development of rapid in vitro assay for oxidative liver injury and its application to 230 chemicals

Abstract

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