TY - JOUR
T1 - Development of radioimmunoassay for human leptin
AU - Hosoda, Kiminori
AU - Masuzaki, Hiroaki
AU - Ogawa, Yoshihiro
AU - Miyawaki, Takashi
AU - Hiraoka, Junko
AU - Hanaoka, Ikuko
AU - Yasuno, Akiko
AU - Nomura, Toshiyuki
AU - Fujisawa, Yukio
AU - Yoshimasa, Yasunao
AU - Nishi, Shigeo
AU - Yamori, Yukio
AU - Nakao, Kazuwa
N1 - Funding Information:
We would like to thank Ms. C. Kawahara for her excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare, Japan Diabetes Foundation, and Uehara Memorial Foundation, and grant for diabetes research from Otuska Pharmaceutical Co., Ltd. (Tokushima, Japan).
PY - 1996/4/16
Y1 - 1996/4/16
N2 - Using recombinant human leptin, we have produced an antiserum for human leptin and developed a radioimmunoassay (RIA) specific and sensitive for human leptin. We detected leptin-like immunoreactivity (-LI) in culture media of adipose tissue from subcutaneous abdominal fat in human. The plasma human leptin-LI concentration in nonobese subjects (17.6 < body mass index (BMI) < 23.0) was 5.6 ± 1.3 (mean ± SE) ng/ml, while that in obese subjects (29.0 < BMI) was 43.0 ± 43.0 ± 9.4 (mean ± SE) ng/ml. In gel permeation chromatographic analyses, leptin-LI in culture media and plasma consisted of single component emerging at the elution position of recombinant human leptin. These findings indicate that leptin is secreted from the adipose tissue into the circulating blood as a large molecular form corresponding to recombinant leptin. The RIA developed in the present study will be a powerful tool to investigate the physiological and pathophysiological significance of leptin in human.
AB - Using recombinant human leptin, we have produced an antiserum for human leptin and developed a radioimmunoassay (RIA) specific and sensitive for human leptin. We detected leptin-like immunoreactivity (-LI) in culture media of adipose tissue from subcutaneous abdominal fat in human. The plasma human leptin-LI concentration in nonobese subjects (17.6 < body mass index (BMI) < 23.0) was 5.6 ± 1.3 (mean ± SE) ng/ml, while that in obese subjects (29.0 < BMI) was 43.0 ± 43.0 ± 9.4 (mean ± SE) ng/ml. In gel permeation chromatographic analyses, leptin-LI in culture media and plasma consisted of single component emerging at the elution position of recombinant human leptin. These findings indicate that leptin is secreted from the adipose tissue into the circulating blood as a large molecular form corresponding to recombinant leptin. The RIA developed in the present study will be a powerful tool to investigate the physiological and pathophysiological significance of leptin in human.
UR - http://www.scopus.com/inward/record.url?scp=0029971425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029971425&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0579
DO - 10.1006/bbrc.1996.0579
M3 - Article
C2 - 8619839
AN - SCOPUS:0029971425
SN - 0006-291X
VL - 221
SP - 234
EP - 239
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -