Development of pH-Responsive BODIPY Probes for Staining Late Endosome in Live Cells

Fang Miao; Shohei Uchinomiya; Yong Ni; Young Tae Chang; Jishan Wu

doi:10.1002/cplu.201600249

Development of pH-Responsive BODIPY Probes for Staining Late Endosome in Live Cells

Fang Miao, Shohei Uchinomiya, Yong Ni, Young Tae Chang, Jishan Wu

Molecular Bioinformatics

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Reported is a new fluorescent probe based on boron–dipyrromethene dye (BODIPY) for late endosome staining in live cells. Among the 14 pH-responsive BODIPY-based far-red/near-infrared dyes used, it was found that a (4-methyl-1-piperazinyl)phenyl-substituted BODIPY could stain late endosomes in three different cell lines. This BODIPY dye was applicable for measurement of the fusion time of late endosomes and lysosomes, thereby demonstrating the utility of this dye for functional analyses of late endosomes in live cells.

Original language	English
Pages (from-to)	1209-1215
Number of pages	7
Journal	ChemPlusChem
Volume	81
Issue number	11
DOIs	https://doi.org/10.1002/cplu.201600249
Publication status	Published - Nov 1 2016

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1002/cplu.201600249

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abstract = "Reported is a new fluorescent probe based on boron–dipyrromethene dye (BODIPY) for late endosome staining in live cells. Among the 14 pH-responsive BODIPY-based far-red/near-infrared dyes used, it was found that a (4-methyl-1-piperazinyl)phenyl-substituted BODIPY could stain late endosomes in three different cell lines. This BODIPY dye was applicable for measurement of the fusion time of late endosomes and lysosomes, thereby demonstrating the utility of this dye for functional analyses of late endosomes in live cells.",

author = "Fang Miao and Shohei Uchinomiya and Yong Ni and Chang, {Young Tae} and Jishan Wu",

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AU - Miao, Fang

AU - Uchinomiya, Shohei

AU - Ni, Yong

AU - Chang, Young Tae

AU - Wu, Jishan

PY - 2016/11/1

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AB - Reported is a new fluorescent probe based on boron–dipyrromethene dye (BODIPY) for late endosome staining in live cells. Among the 14 pH-responsive BODIPY-based far-red/near-infrared dyes used, it was found that a (4-methyl-1-piperazinyl)phenyl-substituted BODIPY could stain late endosomes in three different cell lines. This BODIPY dye was applicable for measurement of the fusion time of late endosomes and lysosomes, thereby demonstrating the utility of this dye for functional analyses of late endosomes in live cells.

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Development of pH-Responsive BODIPY Probes for Staining Late Endosome in Live Cells

Abstract

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