TY - JOUR
T1 - Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles
AU - Tsukamoto, A.
AU - Saitoh, K.
AU - Suzuki, D.
AU - Sugita, N.
AU - Seki, Y.
AU - Kandori, A.
AU - Tsukada, K.
AU - Sugiura, Y.
AU - Hamaoka, S.
AU - Kuma, H.
AU - Hamasaki, N.
AU - Enpuku, K.
N1 - Funding Information:
Manuscript received October 4, 2004. This work was supported by the Ministry of Economy, Trade and Industry for the Regional Renaissance Consortium R&D Program. A. Tsukamoto and K. Saitoh are with the Advanced Research Laboratory, Hitachi, Ltd, Kokubunji-shi, Tokyo 185-8601, Japan (e-mail: tsukamot@ rd.hitachi.co.jp). D. Suzuki, N. Sugita, Y. Seki, and A. Kandori are with the Central Research Laboratory, Hitachi, Ltd, Kokubunji-shi, Tokyo 185-8601, Japan. K. Tsukada is with the Central Research Laboratory, Hitachi, Ltd, Kokubunji-shi, Tokyo 185-8601, Japan and also with the Department of Electrical and Electronic Engineering, Okayama University, Okayama 700-8530, Japan. Y. Sugiura is with the Plastic Products Division, INOAC Corporation, Nagoya 456-0054, Japan. S. Hamaoka is with the Department of Marketing, Kyushu INOAC, Fukuoka 812-0013, Japan. H. Kuma and N. Hamasaki are with the Department of Clinical Chemistry and Laboratory Medicine, Kyushu University, Fukuoka 812-8582, Japan. K. Enpuku is with the Research Institute of Superconductor Science and Systems, Kyushu University, Fukuoka 812-8581, Japan. Digital Object Identifier 10.1109/TASC.2005.849988
PY - 2005/6
Y1 - 2005/6
N2 - We developed a prototype magnetic immunoassay system using a high temperature superconductor (HTS) superconducting quantum interference device (SQUID) to investigate the performance and usability of the magnetic immunoassay. The system is designed to measure multiple samples and liquid samples, and it can work in an unshielded environment at a medical facility. To reduce the disturbance from environmental noise, the SQUID and samples are covered with three-layers of permalloy magnetic shield. The SQUID and magnetic shield are set in an aluminum box which acts as an RF shield. A gradiometer with a 5 × 10 mm pickup coil, which is cooled by liquid nitrogen through a sapphire/Cu rod, is used as a sensor. We also developed a nonmagnetic sample disk with 12 reaction cells and examined 12 samples in one measurement sequence. The measurement process is controlled by a computer, which perform data averaging. Fe3O4 nanoparticles with a 25-nm diameter were used as test samples. After applying a magnetic field of about 0.1 T, we measured the remanent magnetic field from the Fe3O4 nanoparticles. The present system could detect 30 pg of Fe3O 4 nanoparticles. This result was obtained by averaging 100 trials under an unshielded laboratory environment. The measurement time for 100 trials was only 100 s.
AB - We developed a prototype magnetic immunoassay system using a high temperature superconductor (HTS) superconducting quantum interference device (SQUID) to investigate the performance and usability of the magnetic immunoassay. The system is designed to measure multiple samples and liquid samples, and it can work in an unshielded environment at a medical facility. To reduce the disturbance from environmental noise, the SQUID and samples are covered with three-layers of permalloy magnetic shield. The SQUID and magnetic shield are set in an aluminum box which acts as an RF shield. A gradiometer with a 5 × 10 mm pickup coil, which is cooled by liquid nitrogen through a sapphire/Cu rod, is used as a sensor. We also developed a nonmagnetic sample disk with 12 reaction cells and examined 12 samples in one measurement sequence. The measurement process is controlled by a computer, which perform data averaging. Fe3O4 nanoparticles with a 25-nm diameter were used as test samples. After applying a magnetic field of about 0.1 T, we measured the remanent magnetic field from the Fe3O4 nanoparticles. The present system could detect 30 pg of Fe3O 4 nanoparticles. This result was obtained by averaging 100 trials under an unshielded laboratory environment. The measurement time for 100 trials was only 100 s.
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U2 - 10.1109/TASC.2005.849988
DO - 10.1109/TASC.2005.849988
M3 - Article
AN - SCOPUS:22044455000
SN - 1051-8223
VL - 15
SP - 656
EP - 659
JO - IEEE Transactions on Applied Superconductivity
JF - IEEE Transactions on Applied Superconductivity
IS - 2 PART I
ER -