Development of mammalian cell-enclosing calcium-alginate hydrogel fibers in a co-flowing stream

Takayuki Takei, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

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38 Citations (Scopus)


A jetting technique in a liquid-liquid co-flowing stream was applied to the preparation of mammalian cell-enclosing calcium-alginate (Ca-alg) hydrogel fibers of several hundred micrometers in cross-sectional diameter. One percent alginate aqueous solution was extruded from needles (270, 480, 940 μm inner diameter) into a co-flowing laminar stream of 100 mM aqueous calcium chloride solution. The extruded alginate solution was stretched by the CaCl 2 solution, which is known as a "jetting process" , and the Ca-alg hydrogel fibers were formed by gelation of the alginate solution through the uptake of calcium ions in the CaCl 2 solution. The cross-sectional diameter of the hydrogel fibers could be controlled from approximately 100-800 μm by changing the velocities of the alginate and CaCl 2 solution, and the inner diameter of the needle. Approximately 95% of bovine carotid artery vascular endothelial cells remained alive after the process of preparing hydrogel fibers in a co-flowing stream, demonstrating that the cell-enclosing process scarcely influences the viability of the enclosed cells.

Original languageEnglish
Pages (from-to)1014-1017
Number of pages4
JournalBiotechnology Journal
Issue number9
Publication statusPublished - Sept 2006

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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