Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Masayasu Taki; Shohei Iyoshi; Akio Ojida; Itaru Hamachi; Yukio Yamamoto

doi:10.1021/ja100714p

Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Masayasu Taki, Shohei Iyoshi, Akio Ojida, Itaru Hamachi, Yukio Yamamoto

Research output: Contribution to journal › Article › peer-review

191 Citations (Scopus)

Abstract

We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of Cu^I present in living cells.

Original language	English
Pages (from-to)	5938-5939
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	132
Issue number	17
DOIs	https://doi.org/10.1021/ja100714p
Publication status	Published - May 5 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja100714p

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abstract = "We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of CuI present in living cells.",

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AU - Taki, Masayasu

AU - Iyoshi, Shohei

AU - Ojida, Akio

AU - Hamachi, Itaru

AU - Yamamoto, Yukio

PY - 2010/5/5

Y1 - 2010/5/5

N2 - We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of CuI present in living cells.

AB - We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of CuI present in living cells.

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JO - Journal of the American Chemical Society

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ER -

Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems

Abstract

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