Development of high-sensitive cytotoxic detection system using HSP70B promoter

K. I. Wada, L. Xu, T. Okano, A. Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The development of a high-sensitive cytotoxic detection system using heat-shock proteins (HSP)70B promoter was discussed. Human liver carcinoma cell line was grown in DMEM at 37 °C in a humidified atmosphere with 5% CO 2. Cell viability was evaluated by quantification of DNA performed by using Cyquant Proliferation Assay kit. Subsequent sequencing revealed that transcriptional starting site was mapped at - 110 bp from the translational starting site in HepG2. These results suggests that the effect of low concentration of cadmium chloride on cytotoxicity could be detected by luciferase analysis using a part of HASP70B promoter.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress


OtherTransactions - 7th World Biomaterials Congress

All Science Journal Classification (ASJC) codes

  • Engineering(all)


Dive into the research topics of 'Development of high-sensitive cytotoxic detection system using HSP70B promoter'. Together they form a unique fingerprint.

Cite this