Development of EST-SSR markers of Ipomoea nil

Tong Ly; Hiroyuki Fukuoka; Asami Otaka; Atsushi Hoshino; Shigeru Iida; Eiji Nitasaka; Nobuyoshi Watanabe; Tsutomu Kuboyama

doi:10.1270/jsbbs.62.99

Development of EST-SSR markers of Ipomoea nil

Tong Ly, Hiroyuki Fukuoka, Asami Otaka, Atsushi Hoshino, Shigeru Iida, Eiji Nitasaka, Nobuyoshi Watanabe, Tsutomu Kuboyama

Biology, Faculty of Science

Research output: Contribution to journal › Comment/debate › peer-review

6 Citations (Scopus)

Abstract

Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Breeding Science
Volume	62
Issue number	1
DOIs	https://doi.org/10.1270/jsbbs.62.99
Publication status	Published - 2012

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Genetics
Plant Science

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title = "Development of EST-SSR markers of Ipomoea nil",

abstract = "Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.",

author = "Tong Ly and Hiroyuki Fukuoka and Asami Otaka and Atsushi Hoshino and Shigeru Iida and Eiji Nitasaka and Nobuyoshi Watanabe and Tsutomu Kuboyama",

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T1 - Development of EST-SSR markers of Ipomoea nil

AU - Ly, Tong

AU - Fukuoka, Hiroyuki

AU - Otaka, Asami

AU - Hoshino, Atsushi

AU - Iida, Shigeru

AU - Nitasaka, Eiji

AU - Watanabe, Nobuyoshi

AU - Kuboyama, Tsutomu

PY - 2012

Y1 - 2012

N2 - Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.

AB - Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.

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Development of EST-SSR markers of Ipomoea nil

Abstract

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