TY - JOUR
T1 - Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure
AU - Yamada, Yuma
AU - Kogure, Kentaro
AU - Nakamura, Yoshio
AU - Inoue, Kaori
AU - Akita, Hidetaka
AU - Nagatsugi, Fumi
AU - Sasaki, Shigeki
AU - Suhara, Tetsuya
AU - Harashima, Hideyoshi
PY - 2005/10
Y1 - 2005/10
N2 - An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.
AB - An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.
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U2 - 10.1248/bpb.28.1939
DO - 10.1248/bpb.28.1939
M3 - Article
C2 - 16204950
AN - SCOPUS:26444618536
SN - 0918-6158
VL - 28
SP - 1939
EP - 1942
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 10
ER -