Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody

Madan Kumar Paudel; Seiichi Sakamoto; Le Van Huy; Hiroyuki Tanaka; Tomofumi Miyamoto; Akihito Takano; Satoshi Morimoto

doi:10.1080/15321819.2016.1273236

Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody

Madan Kumar Paudel, Seiichi Sakamoto, Le Van Huy, Hiroyuki Tanaka, Tomofumi Miyamoto, Akihito Takano, Satoshi Morimoto

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn–bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.

Original language	English
Pages (from-to)	457-470
Number of pages	14
Journal	Journal of Immunoassay and Immunochemistry
Volume	38
Issue number	5
DOIs	https://doi.org/10.1080/15321819.2016.1273236
Publication status	Published - Sept 3 2017

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Clinical Biochemistry
Medical Laboratory Technology

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10.1080/15321819.2016.1273236

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title = "Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody",

abstract = "Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn–bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.",

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AU - Paudel, Madan Kumar

AU - Sakamoto, Seiichi

AU - Huy, Le Van

AU - Tanaka, Hiroyuki

AU - Miyamoto, Tomofumi

AU - Takano, Akihito

AU - Morimoto, Satoshi

PY - 2017/9/3

Y1 - 2017/9/3

N2 - Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn–bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.

AB - Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn–bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.

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Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody

Abstract

All Science Journal Classification (ASJC) codes

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