TY - JOUR
T1 - Development of an enzyme linked immunosorbent assay for direct determination of anticancer drug vitamin K3 in serum
AU - Sakamoto, Seiichi
AU - Sakoda, Junichi
AU - Morinaga, Osamu
AU - Waraporn, Putalun
AU - Tsuchihashi, Ryota
AU - Morimoto, Satoshi
AU - Kinjo, Junei
AU - Tanaka, Hiroyuki
PY - 2008/8
Y1 - 2008/8
N2 - Synthetic vitamin K3 (VK3, 2-methyl-1,4-naphthoquinone, or menadione) has been shown to have anticancer activity in various human cancer cells. We developed an enzyme-linked immunosorbant assay (ELISA) for determination of VK3 in fetal calf serum (FCS) without any pretreatment. The monoclonal antibody against VK3, which was secreted from an established hybridoma cell line (3A3) has been proven to have highly specific binding to VK3 in cross-reactivity analysis. The full measuring range of the assay extends from 0.39 to 50 μg/ml of VK3. Based on validation analysis, this immunological analysis is a precise, accurate, and sensitive method for determination of VK3. This simple immunological method would deserve to be applicable clinically.
AB - Synthetic vitamin K3 (VK3, 2-methyl-1,4-naphthoquinone, or menadione) has been shown to have anticancer activity in various human cancer cells. We developed an enzyme-linked immunosorbant assay (ELISA) for determination of VK3 in fetal calf serum (FCS) without any pretreatment. The monoclonal antibody against VK3, which was secreted from an established hybridoma cell line (3A3) has been proven to have highly specific binding to VK3 in cross-reactivity analysis. The full measuring range of the assay extends from 0.39 to 50 μg/ml of VK3. Based on validation analysis, this immunological analysis is a precise, accurate, and sensitive method for determination of VK3. This simple immunological method would deserve to be applicable clinically.
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U2 - 10.1248/jhs.54.508
DO - 10.1248/jhs.54.508
M3 - Article
AN - SCOPUS:48749103256
SN - 1344-9702
VL - 54
SP - 508
EP - 511
JO - Journal of Health Science
JF - Journal of Health Science
IS - 4
ER -
