Development of an effective protein-labeling system based on smart fluorogenic probes

Shahi Imam Reja, Masafumi Minoshima, Yuichiro Hori, Kazuya Kikuchi

Research output: Contribution to journalReview articlepeer-review

3 Citations (Scopus)


Abstract: Proteins are an important component of living systems and play a crucial role in various physiological functions. Fluorescence imaging of proteins is a powerful tool for monitoring protein dynamics. Fluorescent protein (FP)-based labeling methods are frequently used to monitor the movement and interaction of cellular proteins. However, alternative methods have also been developed that allow the use of synthetic fluorescent probes to target a protein of interest (POI). Synthetic fluorescent probes have various advantages over FP-based labeling methods. They are smaller in size than the fluorescent proteins, offer a wide variety of colors and have improved photochemical properties. There are various chemical recognition-based labeling techniques that can be used for labeling a POI with a synthetic probe. In this review, we focus on the development of protein-labeling systems, particularly the SNAP-tag, BL-tag, and PYP-tag systems, and understanding the fluorescence behavior of the fluorescently labeled target protein in these systems. We also discuss the smart fluorogenic probes for these protein-labeling systems and their applications. The fluorogenic protein labeling will be a useful tool to investigate complex biological phenomena in future work on cell biology. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)443-455
Number of pages13
JournalJournal of Biological Inorganic Chemistry
Issue number4
Publication statusPublished - Jun 1 2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry


Dive into the research topics of 'Development of an effective protein-labeling system based on smart fluorogenic probes'. Together they form a unique fingerprint.

Cite this