TY - JOUR
T1 - Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator
AU - Ijima, Hiroyuki
AU - Kakeya, Yasuo
AU - Ogata, Takahiro
AU - Sakai, Takanori
N1 - Funding Information:
This work was supported in part by Grant-in-Aids for Scientific Research (B): 18360399 from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by Fukuoka Biotechnology Basic Technology Development Project from Fukuoka Prefecture. The authors express their sincere appreciation for Mr. Masahiro Miura of Kyushu University for manufacturing the device.
PY - 2009/5/15
Y1 - 2009/5/15
N2 - We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23% lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown
AB - We developed an easy-to-use, small-scale circulation-type bioreactor system that enables the simultaneous evaluation of many specimens. Medium flow was generated by a magnetic stirrer in this system. Primary rat hepatocytes formed a monolayer, and there were no morphological differences between cells in circulation and stationary cultures. The mitochondrial activity of hepatocytes in the circulation culture was 23% lower than that in the stationary culture after 2 days of culture. On the other hand, albumin production activity in the circulation culture after 2 days of culture was 1.4 times higher than that in the stationary culture. Albumin production activity per cell in the circulation culture was 1.9 times higher than that in the stationary culture after 2 days of culture. In addition, lidocaine metabolism rate per cell in the circulation culture was 1.3 times higher than that in the stationary culture. The lidocaine clearance of the circulation culture in our circulation-type bioreactor was 1.3 times higher than that of the stationary culture. It was shown that this bioreactor is suitable for the expression of the liver-specific functions of primary rat hepatocytes. Therefore, we can expect that this circulation-type bioreactor system will be a practical drug metabolism simulator. Crown
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U2 - 10.1016/j.bej.2008.12.015
DO - 10.1016/j.bej.2008.12.015
M3 - Article
AN - SCOPUS:61449203717
SN - 1369-703X
VL - 44
SP - 292
EP - 296
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 2-3
ER -