Development of a novel polycationic adsorbent for cryogel removal

Keiichi Miyamoto, Ryuji Uchiyama, Masayuki Tokita, Motoki Yonekawa, Akio Kawamura, Keiichi Miyashita, Eiji Sakashita, Takashi Komai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Cryogel, prevalent in the plasma of rheumatism patients, is a plasma fibronectin (pFN)-extra domain A containing FN (EDA(+)FN)-fibrinogen (Fbg) complex formed by adding heparin (HP) at a low temperature (4°C). Although EDA(+)FN does not usually exist in normal plasma, its prevalence in rheumatic patients causes cryogelation in plasma. Removal of cryogel is thus a promising and novel approach to treating rheumatism. As HP-EDA(+)FN aggregate, which is induced by the main component of cryogel, is considered to be an anion, cationic materials capable of eliminating this anionic conjugate were innovated in this study. We found that an amino group density of 100-130 μmol/g (dry weight) of adsorbents prompted selective adsorption of the EDA(+)FN-HP complex. Elimination of EDA(+)FN as high as 80% accompanied by removal of the components of total FN (pFN) (10%) and Fbg (10%) in the model patient plasma was established.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalInternational Journal of Biological Macromolecules
Issue number1
Publication statusPublished - Jul 19 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Structural Biology
  • Biochemistry


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