Development of a novel immobilization method for enzymes from hyperthermophiles

Kosuke Minamihata, Masamichi Tokunaga, Noriho Kamiya, Shiro Kiyoyama, Haruhiko Sakuraba, Toshihisa Ohshima, Masahiro Goto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Peptide tags containing tyrosines (Y-tag) were introduced at the C-terminus of a hyperthermophilic enzyme, alkaline phosphatase from Pyrococcus furiosus (PfuAP). Immobilization of the recombinant PfuAPs onto water-in-oil-in-water (W/O/W) type microcapsules was performed by an in situ polymerization method. All the recombinant PfuAPs prepared in this study were quantitatively immobilized onto microcapsules. The PfuAP-immobilized microcapsules showed no significant loss of enzymatic activity until the 5th round of assays. This result implies that the recombinant PfuAPs were covalently immobilized onto microcapsules. Immobilized PfuAP tagged with a Y-tag having the sequence GGYYY exhibited approximately a twofold higher catalytic activity compared with the wild-type PfuAP.

Original languageEnglish
Pages (from-to)1037-1041
Number of pages5
JournalBiotechnology letters
Issue number7
Publication statusPublished - Jul 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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