TY - JOUR
T1 - Development of a new mouse model of branch retinal vein occlusion and retinal neovascularization
AU - Zhang, Han
AU - Sonoda, Koh Hei
AU - Qiao, Hong
AU - Oshima, Toru
AU - Hisatomi, Toshio
AU - Ishibashi, Tatsuro
N1 - Funding Information:
Acknowledgments. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (B2 No. 14770962 to KS, C2 No. 16591757 to KS). The authors thank Ms. Mari Imamura, Ms. Michiyo Takahara, and Ms. Fumiyo Morikawa for their excellent technical assistance.
PY - 2007/7
Y1 - 2007/7
N2 - Purpose: Retinal neovascularization (NV) is associated with various disorders, such as retinal vein occlusion, diabetic retinopathy, and retinopathy of prematurity, and often causes severe loss of vision. To determine the mechanism of retinal NV and develop new therapy, we developed a mouse model using a photodynamic method. Methods: C57BL/6 mice were injected with rose bengal via the tail vein, and then selected venous points were photocoagulated. Results: All eyes demonstrated venous occlusion on day 1, and capillary nonperfusion areas were observed until day 3. Twenty of 33 eyes (60.6%) developed retinal NV on day 14, confirmed by fluorescein isothiocyanate-perfused retinal flat-mounts and immunochemical and histopathological analyses. Reverse transcriptase-polymerase chain reaction showed an increase in the expression of vascular endothelial growth factor at the retina on day 7. Conclusions: Because of the simplicity, low cost, and feasibility of genetic manipulations, our model is believed to represent an advance in investigating molecular mechanisms and establishing therapy for retinal NV.
AB - Purpose: Retinal neovascularization (NV) is associated with various disorders, such as retinal vein occlusion, diabetic retinopathy, and retinopathy of prematurity, and often causes severe loss of vision. To determine the mechanism of retinal NV and develop new therapy, we developed a mouse model using a photodynamic method. Methods: C57BL/6 mice were injected with rose bengal via the tail vein, and then selected venous points were photocoagulated. Results: All eyes demonstrated venous occlusion on day 1, and capillary nonperfusion areas were observed until day 3. Twenty of 33 eyes (60.6%) developed retinal NV on day 14, confirmed by fluorescein isothiocyanate-perfused retinal flat-mounts and immunochemical and histopathological analyses. Reverse transcriptase-polymerase chain reaction showed an increase in the expression of vascular endothelial growth factor at the retina on day 7. Conclusions: Because of the simplicity, low cost, and feasibility of genetic manipulations, our model is believed to represent an advance in investigating molecular mechanisms and establishing therapy for retinal NV.
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U2 - 10.1007/s10384-007-0445-2
DO - 10.1007/s10384-007-0445-2
M3 - Article
C2 - 17660984
AN - SCOPUS:34547398547
SN - 0021-5155
VL - 51
SP - 251
EP - 257
JO - Japanese Journal of Ophthalmology
JF - Japanese Journal of Ophthalmology
IS - 4
ER -
