TY - JOUR
T1 - Development of a microbioreactor for degradation of environmental pollutants
AU - Michizoe, Junji
AU - Shinohara, Kenji
AU - Maruyama, Tatsuo
AU - Kusakabe, Katsuki
AU - Maeda, Hideaki
AU - Goto, Masahiro
PY - 2003/1
Y1 - 2003/1
N2 - A microbioreactor (upper width = 433 μm, lower width = 296 μm, depth = 91 μm) was designed on a silicon wafer by wet chemical etching, and the resulting channel was seated with a glass cover by an anodic bonding technique. Biodegradation of an environmental pollutant (p-chlorophenol) was conducted using laccase in the microreactor. The enzymatic reaction smoothly proceeded in the microreactor, and the reaction rate was enhanced 400-fold compared to that of an ordinary bulk reaction. It was found that the enzyme exhibited high stability in the microreactor, which allows recycling the enzyme solution. The biodegradation yield attained 90% when several microreactors were connected in series.
AB - A microbioreactor (upper width = 433 μm, lower width = 296 μm, depth = 91 μm) was designed on a silicon wafer by wet chemical etching, and the resulting channel was seated with a glass cover by an anodic bonding technique. Biodegradation of an environmental pollutant (p-chlorophenol) was conducted using laccase in the microreactor. The enzymatic reaction smoothly proceeded in the microreactor, and the reaction rate was enhanced 400-fold compared to that of an ordinary bulk reaction. It was found that the enzyme exhibited high stability in the microreactor, which allows recycling the enzyme solution. The biodegradation yield attained 90% when several microreactors were connected in series.
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U2 - 10.1252/kakoronbunshu.29.82
DO - 10.1252/kakoronbunshu.29.82
M3 - Article
AN - SCOPUS:0037246458
SN - 0386-216X
VL - 29
SP - 82
EP - 86
JO - kagaku kogaku ronbunshu
JF - kagaku kogaku ronbunshu
IS - 1
ER -