TY - JOUR
T1 - Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin
AU - Krittanai, Supaluk
AU - Kitisripanya, Tharita
AU - Udomsin, Orapin
AU - Tanaka, Hiroyuki
AU - Sakamoto, Seiichi
AU - Juengwatanatrakul, Thaweesak
AU - Putalun, Waraporn
N1 - Funding Information:
This research was supported by the Faculty of Pharmaceutical Sciences, Graduate School of Khon Kaen University, Thailand (grant number 590H11) and the National Research Council of Thailand. The authors would like to express gratitude to Dr Chaiyo Chaichantipyuth, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand, for an authentic standard of miroestrol.
Publisher Copyright:
© 2018 John Wiley & Sons, Ltd.
PY - 2018/11
Y1 - 2018/11
N2 - Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.
AB - Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.
UR - http://www.scopus.com/inward/record.url?scp=85052482177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052482177&partnerID=8YFLogxK
U2 - 10.1002/bmc.4330
DO - 10.1002/bmc.4330
M3 - Article
C2 - 29972702
AN - SCOPUS:85052482177
SN - 0269-3879
VL - 32
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 11
M1 - e4330
ER -