TY - GEN
T1 - Development of μtas for high-throughput screening of nad(p)-dependent oxidoreductases
AU - Shimokihara, Shota
AU - Shitara, Shunya
AU - Oyobiki, Ryo
AU - Watanabe, Takeshi
AU - Einaga, Yasuaki
AU - Matsumoto, Yoshinori
AU - Fujiwara, Kei
AU - Horisawa, Kenichi
AU - Doi, Nobuhide
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.
AB - Although oxidoreductases are widely used in many applications, such as biosensors and biofuel cells, improvements in the function of existing oxidoreductases or the discovery of novel oxidoreductases with greater activity is desired. To increase the activity of oxidoreductases by directed evolution, a powerful screening technique for oxidoreductases is required. In this study, we developed a microfluidic total analysis system for high-throughput screening of NAD(P)-dependent oxidoreductases. This system comprises three microfluidic devices: an encapsulating device, a NADH measuring device, and a magnetic beads sorting device. We demonstrated the utility of each part of the system.
UR - http://www.scopus.com/inward/record.url?scp=84983317467&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983317467&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983317467
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1302
EP - 1304
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -