Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

E. Fukatsu; K. Isoda; T. Hirao; M. Takahashi; A. Watanabe

doi:10.1111/j.1471-8286.2005.00933.x

Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

E. Fukatsu, K. Isoda, T. Hirao, M. Takahashi, A. Watanabe

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.

Original language	English
Pages (from-to)	378-380
Number of pages	3
Journal	Molecular Ecology Notes
Volume	5
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00933.x
Publication status	Published - Jun 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2005.00933.x

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abstract = "We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.",

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AU - Isoda, K.

AU - Hirao, T.

AU - Takahashi, M.

AU - Watanabe, A.

PY - 2005/6

Y1 - 2005/6

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AB - We developed 14 microsatellite loci from an enriched genomic DNA library of a broad-leaved deciduous tree, Zelkova serrata. Of 198 clones from the library, 112 contained microsatellite repeat regions. The M13-tailed primer method was used for economy. Sequence-specific primer pairs were designed for 58 of 76 candidate clones. Fourteen of these primer pairs successfully amplified polymorphic single loci among 34 individuals collected from the Kanto breeding region in Japan. The expected heterozygosity for the 14 microsatellite markers ranged from 0.378 to 0.876, suggesting that these will prove valuable for breeding and ecological studies on Z. serrata.

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Development and characterization of simple sequence repeat DNA markers for Zelkova serrata

Abstract

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