Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet

Azizah Intan Pangesty, Takaaki Arahira, Mitsugu Todo

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    12 Citations (Scopus)


    Abstract: Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties. Graphical abstract: [InlineMediaObject not available: see fulltext.].

    Original languageEnglish
    Article number165
    JournalJournal of Materials Science: Materials in Medicine
    Issue number10
    Publication statusPublished - Oct 1 2017

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Biomedical Engineering


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