TY - JOUR
T1 - Detoxification roles of tributyltin-binding protein type 2 in Japanese medaka (Oryzias latipes) exposed to tributyltin
AU - Qiu, Xuchun
AU - Takamura, Takumi
AU - Enoki, Shintaro
AU - Kato-Unoki, Yoko
AU - Takai, Yuki
AU - Nagano, Yosuke
AU - Kinoshita, Masato
AU - Kitano, Takeshi
AU - Shimasaki, Yohei
AU - Oshima, Yuji
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science KAKENHI (Grant number JP18H02280 ).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - Tributyltin-binding protein type 2 (TBT-bp2), a homolog of α1-acid glycoprotein, may contribute to both accumulation and detoxification of TBT in fish. In this study, we conducted acute TBT exposure tests using both wide-type (WT) and TBT-bp2−/− (KO) strains of medaka and compared their responses in survival time and accumulation of TBT. Deficiency of TBT-bp2 significantly accelerated the time to death of medaka and decreased the LC50 of TBT, indicating that the KO-strain is more sensitive to TBT. No significant difference in the intrinsic TBT concentration in surviving fish was observed between the two strains. However, the intrinsic TBT concentration in dead KO-strain was significantly lower than that in WT-strain. These findings provide direct evidence, supporting the hypothesis that TBT-bp2 plays a critical role in the detoxification of TBT in fish.
AB - Tributyltin-binding protein type 2 (TBT-bp2), a homolog of α1-acid glycoprotein, may contribute to both accumulation and detoxification of TBT in fish. In this study, we conducted acute TBT exposure tests using both wide-type (WT) and TBT-bp2−/− (KO) strains of medaka and compared their responses in survival time and accumulation of TBT. Deficiency of TBT-bp2 significantly accelerated the time to death of medaka and decreased the LC50 of TBT, indicating that the KO-strain is more sensitive to TBT. No significant difference in the intrinsic TBT concentration in surviving fish was observed between the two strains. However, the intrinsic TBT concentration in dead KO-strain was significantly lower than that in WT-strain. These findings provide direct evidence, supporting the hypothesis that TBT-bp2 plays a critical role in the detoxification of TBT in fish.
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U2 - 10.1016/j.marpolbul.2020.111445
DO - 10.1016/j.marpolbul.2020.111445
M3 - Article
C2 - 32758797
AN - SCOPUS:85088935784
SN - 0025-326X
VL - 159
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
M1 - 111445
ER -