TY - JOUR
T1 - Determination of temporal changes in serum and urinary lactate and 3-hydroxybutyrate enantiomers in mice with nephrotoxic serum nephritis by multi-dimensional HPLC
AU - Lin, Po Yeh
AU - Chen, Shih Ming
AU - Hsieh, Chin Ling
AU - Lin, Chia Yu
AU - Huang, Yu Sheng
AU - Hamase, Kenji
AU - Lee, Jen Ai
N1 - Funding Information:
We appreciate that Professor Ueda Shiro supplied our team the antiserum. This work was financially supported by the Ministry of Science and Technology of the Republic of China ( MOST 105-2320-B-038-025 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9/5
Y1 - 2020/9/5
N2 - Nephrotoxic serum (NTS) nephritis occurs in three stages: inflammation, early kidney damage, and severe kidney damage. We quantified the temporal changes in the enantiomers of lactate (LA) and 3-hydroxybutyrate (3HB) in serum and urine during the progression of autoimmune kidney damage in mice with NTS nephritis. Two-dimensional and three-dimensional HPLC were used to quantify the enantiomers. The serum and urinary levels of LA and 3HB enantiomers significantly changed during the progression of NTS nephritis. Specifically, D-LA was significantly higher in the serum (131.8 ± 30.6, 123.7 ± 27.2, 109.3 ± 15.6 vs. 51.2 ± 7.5 μM; p < 0.05) and urine (222.2 ± 34.8, 197.4 ± 53.9, 214.8 ± 68.9 vs. 100.8 ± 37.7 μmol/g creatinine; p < 0.05) of the week 0 (W0), week 1 (W1), and week 2 (W2) groups than the normal group. The L-3HB/D-3HB ratio was significantly lower in the W0, W1, and W2 groups than the normal group in serum (0.0362 ± 0.0082, 0.0346 ± 0.0065, 0.0323 ± 0.0033 vs. 0.0602 ± 0.0214; p < 0.05) and urine (0.0591 ± 0.0304, 0.1524 ± 0.0365, 0.1232 ± 0.1066 vs. 0.3273 ± 0.1394; p < 0.05). The changes in serum and urinary D-LA and L-3HB/D-3HB ratios were stable before severe kidney damage. In conclusion, we successfully determined the levels of LA and 3HB enantiomers in NTS nephritis by HPLC. Serum and urinary D-LA contents and L-3HB/D-3HB ratios may have potential as biomarkers of early autoimmune kidney injury.
AB - Nephrotoxic serum (NTS) nephritis occurs in three stages: inflammation, early kidney damage, and severe kidney damage. We quantified the temporal changes in the enantiomers of lactate (LA) and 3-hydroxybutyrate (3HB) in serum and urine during the progression of autoimmune kidney damage in mice with NTS nephritis. Two-dimensional and three-dimensional HPLC were used to quantify the enantiomers. The serum and urinary levels of LA and 3HB enantiomers significantly changed during the progression of NTS nephritis. Specifically, D-LA was significantly higher in the serum (131.8 ± 30.6, 123.7 ± 27.2, 109.3 ± 15.6 vs. 51.2 ± 7.5 μM; p < 0.05) and urine (222.2 ± 34.8, 197.4 ± 53.9, 214.8 ± 68.9 vs. 100.8 ± 37.7 μmol/g creatinine; p < 0.05) of the week 0 (W0), week 1 (W1), and week 2 (W2) groups than the normal group. The L-3HB/D-3HB ratio was significantly lower in the W0, W1, and W2 groups than the normal group in serum (0.0362 ± 0.0082, 0.0346 ± 0.0065, 0.0323 ± 0.0033 vs. 0.0602 ± 0.0214; p < 0.05) and urine (0.0591 ± 0.0304, 0.1524 ± 0.0365, 0.1232 ± 0.1066 vs. 0.3273 ± 0.1394; p < 0.05). The changes in serum and urinary D-LA and L-3HB/D-3HB ratios were stable before severe kidney damage. In conclusion, we successfully determined the levels of LA and 3HB enantiomers in NTS nephritis by HPLC. Serum and urinary D-LA contents and L-3HB/D-3HB ratios may have potential as biomarkers of early autoimmune kidney injury.
UR - http://www.scopus.com/inward/record.url?scp=85085893242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85085893242&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2020.113367
DO - 10.1016/j.jpba.2020.113367
M3 - Article
C2 - 32512253
AN - SCOPUS:85085893242
SN - 0731-7085
VL - 188
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
M1 - 113367
ER -