TY - JOUR
T1 - Determination of nerve agent metabolites in human urine by femtosecond laser ionization mass spectrometry using 2-(bromomethyl)naphthalene as a derivatizing reagent
AU - Son, Vu Van
AU - Nakamura, Hiroshi
AU - Imasaka, Tomoko
AU - Imasaka, Totaro
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [JSPS KAKENHI Grant Numbers JP26220806 ] and by the Program of Progress 100 in Kyushu University . Quantum chemical calculations were mainly carried out using the computer facilities at the Research Institute for Information Technology, Kyushu University.
Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [JSPS KAKENHI Grant Numbers JP26220806] and by the Program of Progress 100 in Kyushu University. Quantum chemical calculations were mainly carried out using the computer facilities at the Research Institute for Information Technology, Kyushu University.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/9/3
Y1 - 2019/9/3
N2 - Nerve agent metabolites (NAMs) derived from alkyl methyl phosphonic acids, such as ethyl methylphosphonic acid (EMPA), isopropyl methylphosphonic acid (IMPA), and pinacolyl methylphosphonic acid (PMPA), were extracted from human urine using diethyl ether as an extractant. After exchanging the diethyl ether solvent to acetonitrile, the analytes were derivatized with 2-(bromomethyl)naphthalene (BMN). The reaction products of the BMN and NAMs, i.e., MN-EMPA, MN-IMPA, and MN-PMPA, were separated by gas chromatography (GC) and measured by mass spectrometry (MS) using a femtosecond laser emitting at 267 nm as the ionization source for resonance-enhanced two-photon ionization (RE2PI). The limits of detection (LOD) were <1 ng/mL for these analytes. The use of BMN increased the volatility of the analytes for separation by GC and also increased the ionization efficiency via the RE2PI process as the result of presence of a naphthalene functional group. A two-dimensional GC-MS display can be used for comprehensive analysis of NAMs, by-products, and impurities in the sample. Then, this approach could be used to confirm the use of chemical weapons and for forensic identification.
AB - Nerve agent metabolites (NAMs) derived from alkyl methyl phosphonic acids, such as ethyl methylphosphonic acid (EMPA), isopropyl methylphosphonic acid (IMPA), and pinacolyl methylphosphonic acid (PMPA), were extracted from human urine using diethyl ether as an extractant. After exchanging the diethyl ether solvent to acetonitrile, the analytes were derivatized with 2-(bromomethyl)naphthalene (BMN). The reaction products of the BMN and NAMs, i.e., MN-EMPA, MN-IMPA, and MN-PMPA, were separated by gas chromatography (GC) and measured by mass spectrometry (MS) using a femtosecond laser emitting at 267 nm as the ionization source for resonance-enhanced two-photon ionization (RE2PI). The limits of detection (LOD) were <1 ng/mL for these analytes. The use of BMN increased the volatility of the analytes for separation by GC and also increased the ionization efficiency via the RE2PI process as the result of presence of a naphthalene functional group. A two-dimensional GC-MS display can be used for comprehensive analysis of NAMs, by-products, and impurities in the sample. Then, this approach could be used to confirm the use of chemical weapons and for forensic identification.
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U2 - 10.1016/j.aca.2019.04.012
DO - 10.1016/j.aca.2019.04.012
M3 - Article
C2 - 31084744
AN - SCOPUS:85064175584
SN - 0003-2670
VL - 1069
SP - 82
EP - 88
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -