Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays

T. Nakahara; A. Shiraishi; M. Hirano; T. Matsumoto; T. Kuroki; Y. Tatebayashi; T. Tsutsumi; K. Nishiyama; H. Ooboshi; K. Nakamura; H. Yao; M. Waki; H. Uchimura

Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays

T. Nakahara, A. Shiraishi, M. Hirano, T. Matsumoto, T. Kuroki, Y. Tatebayashi, T. Tsutsumi, K. Nishiyama, H. Ooboshi, K. Nakamura, H. Yao, M. Waki, H. Uchimura

Clinical Psychology Practice

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Abstract

A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.

Original language	English
Pages (from-to)	38-42
Number of pages	5
Journal	Analytical Biochemistry
Volume	180
Issue number	1
Publication status	Published - 1989

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

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title = "Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays",

abstract = "A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.",

author = "T. Nakahara and A. Shiraishi and M. Hirano and T. Matsumoto and T. Kuroki and Y. Tatebayashi and T. Tsutsumi and K. Nishiyama and H. Ooboshi and K. Nakamura and H. Yao and M. Waki and H. Uchimura",

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T1 - Determination of guanine by high-performance liquid chromatography with electrochemical detection

T2 - Application to DNA and RNA assays

AU - Nakahara, T.

AU - Shiraishi, A.

AU - Hirano, M.

AU - Matsumoto, T.

AU - Kuroki, T.

AU - Tatebayashi, Y.

AU - Tsutsumi, T.

AU - Nishiyama, K.

AU - Ooboshi, H.

AU - Nakamura, K.

AU - Yao, H.

AU - Waki, M.

AU - Uchimura, H.

PY - 1989

Y1 - 1989

N2 - A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.

AB - A highly sensitive assay for guanine was developed using high-performance liquid chromatography with electrochemical detection (ECD). Guanine was susceptible to the electrochemical oxidation, and ECD response was proportional to the amount of guanine in the range 0.25-4 pmol of guanine. The ECD of guanine was applicable to the analysis of nucleic acids. DNA and RNA were hydrolyzed in 0.03 and 3 M HCl, respectively, and guanine liberated from the nucleic acids was separated on a reverse-phase column and determined by ECD. The method allowed detection of 0.2 ng of calf thymus DNA or tRNA. An application of the method is shown for DNA and RNA assays in trichloroacetic acid extracts of rat adrenal and liver.

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Determination of guanine by high-performance liquid chromatography with electrochemical detection: Application to DNA and RNA assays

Abstract

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