Determination of Fragrance Allergens by Ultraviolet Femtosecond Laser Ionization Mass Spectrometry

Shimpei Shibuta, Tomoko Imasaka, Totaro Imasaka

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    20 Citations (Scopus)


    The allergenic compounds listed in the Cosmetics Directive by the Scientific Committee for Consumer Safety were analyzed by gas chromatography combined with multiphoton ionization mass spectrometry using a femtosecond laser emitting at 200 and 267 nm as the ionization source. The limits of detection were less than 100 pg/μL for all of the compounds, permitting them to be measured in actual samples that were simply prepared by a 100-fold dilution of the original sample. The ionization process was investigated for the 26 allergens, some of which had no absorption band, even in the far-UV region. As a result, nonresonant two-photon ionization was found to be the most sensitive and universal method for the trace analysis of these compounds, because of the short pulse width, i.e., a high peak power, of the femtosecond laser used. It should be noted that the excess energy can be reduced by using a laser emitting at longer wavelengths (267 nm) and that fragmentation can be suppressed, especially for a molecule that contains a long side chain. Three commercially available perfumes were measured, and more than 10 allergenic compounds were determined.

    Original languageEnglish
    Pages (from-to)10693-10700
    Number of pages8
    JournalAnalytical Chemistry
    Issue number21
    Publication statusPublished - Nov 1 2016

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry


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