Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

Shigetoshi Kage; Keiko Kudo; Naoki Nishida; Hideaki Ikeda; Naofumi Yoshioka; Noriaki Ikeda

doi:10.1007/s11419-008-0043-0

Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

Shigetoshi Kage, Keiko Kudo, Naoki Nishida, Hideaki Ikeda, Naofumi Yoshioka, Noriaki Ikeda

Social Medicine

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25 Citations (Scopus)

Abstract

We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

Original language	English
Pages (from-to)	23-26
Number of pages	4
Journal	Forensic Toxicology
Volume	26
Issue number	1
DOIs	https://doi.org/10.1007/s11419-008-0043-0
Publication status	Published - Jun 2008

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Toxicology
Biochemistry, medical

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10.1007/s11419-008-0043-0

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abstract = "We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.",

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T1 - Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

AU - Kage, Shigetoshi

AU - Kudo, Keiko

AU - Nishida, Naoki

AU - Ikeda, Hideaki

AU - Yoshioka, Naofumi

AU - Ikeda, Noriaki

PY - 2008/6

Y1 - 2008/6

N2 - We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

AB - We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry (GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

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Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

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