Determination of bisphosphonates by ion-pair HPLC

Łukasz Matuszewski, Anna Matuszewska, Tomasz Mazurkiewicz, Jerzy Rogalski, Nam Seok Cho, Shoji Ohga

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10-7-10-8g/ml). It can be easily and conveniently adopted for the routine quality control analysis.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalJournal of the Faculty of Agriculture, Kyushu University
Issue number2
Publication statusPublished - Sept 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science


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