Determination of antihypertensive small peptides, Val-Tyr and Ile-Val-Tyr, by fluorometric high-performance liquid chromatography combined with a double heart-cut column-switching technique

Takao Ueno; Mitsuru Tanaka; Toshiro Matsui; Kiyoshi Matsumoto

doi:10.2116/analsci.21.997

Determination of antihypertensive small peptides, Val-Tyr and Ile-Val-Tyr, by fluorometric high-performance liquid chromatography combined with a double heart-cut column-switching technique

Takao Ueno, Mitsuru Tanaka, Toshiro Matsui, Kiyoshi Matsumoto

Food Science and Biotechnology

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14 Citations (Scopus)

Abstract

A double column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was applied for determination of two plasma antihypertensive peptides, Val-Tyr (VY) and Ile-Val-Tyr (IVY). After a first separation on a Phe-ODS column, double heart-cuts of the retention time corresponding to NDA-VY and NDA-IVY elutions were successfully separated on an analytical ODS column: 60% acetonitrile in 0.1% trifluoroacetic acid containing 5 mM sodium 1-octanesulfonate at 1.0 mL/min. Within-run coefficients of variation were 1.73% and 4.73% for VY and IVY, respectively. 2005

Original language	English
Pages (from-to)	997-1000
Number of pages	4
Journal	analytical sciences
Volume	21
Issue number	8
DOIs	https://doi.org/10.2116/analsci.21.997
Publication status	Published - Aug 2005

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.2116/analsci.21.997

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Determination of antihypertensive small peptides, Val-Tyr and Ile-Val-Tyr, by fluorometric high-performance liquid chromatography combined with a double heart-cut column-switching technique. / Ueno, Takao; Tanaka, Mitsuru ; Matsui, Toshiro et al.
In: analytical sciences, Vol. 21, No. 8, 08.2005, p. 997-1000.

Research output: Contribution to journal › Article › peer-review

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abstract = "A double column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was applied for determination of two plasma antihypertensive peptides, Val-Tyr (VY) and Ile-Val-Tyr (IVY). After a first separation on a Phe-ODS column, double heart-cuts of the retention time corresponding to NDA-VY and NDA-IVY elutions were successfully separated on an analytical ODS column: 60% acetonitrile in 0.1% trifluoroacetic acid containing 5 mM sodium 1-octanesulfonate at 1.0 mL/min. Within-run coefficients of variation were 1.73% and 4.73% for VY and IVY, respectively. 2005",

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AU - Matsui, Toshiro

AU - Matsumoto, Kiyoshi

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Determination of antihypertensive small peptides, Val-Tyr and Ile-Val-Tyr, by fluorometric high-performance liquid chromatography combined with a double heart-cut column-switching technique

Abstract

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