Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Waraporn Putalun; Hiroyuki Tanaka; Toshiya Muranaka; Yukihiro Shoyama

doi:10.1039/b203351h

Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Waraporn Putalun, Hiroyuki Tanaka, Toshiya Muranaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

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16 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for determination of aculeatisides. Aculeatiside A was conjugated with bovine serum albumin (BSA) for immunization. The ratio of hapten in an antigen conjugate was determined by matrix-assisted laser desorption/ionization TOF mass spectrometry. Polyclonal antibody was developed in rabbits against an aculeatiside A-BSA conjugate. The antibody was specific for aculeatiside A and aculeatiside B. The range of the immunoassay extended from 100 ng ml^-1 to 5 μg ml^-1 of aculeatisides. Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples.

Original language	English
Pages (from-to)	1328-1332
Number of pages	5
Journal	Analyst
Volume	127
Issue number	10
DOIs	https://doi.org/10.1039/b203351h
Publication status	Published - Oct 2002

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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title = "Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A",

abstract = "An enzyme-linked immunosorbent assay (ELISA) was developed for determination of aculeatisides. Aculeatiside A was conjugated with bovine serum albumin (BSA) for immunization. The ratio of hapten in an antigen conjugate was determined by matrix-assisted laser desorption/ionization TOF mass spectrometry. Polyclonal antibody was developed in rabbits against an aculeatiside A-BSA conjugate. The antibody was specific for aculeatiside A and aculeatiside B. The range of the immunoassay extended from 100 ng ml-1 to 5 μg ml-1 of aculeatisides. Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples.",

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T1 - Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

AU - Putalun, Waraporn

AU - Tanaka, Hiroyuki

AU - Muranaka, Toshiya

AU - Shoyama, Yukihiro

PY - 2002/10

Y1 - 2002/10

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AB - An enzyme-linked immunosorbent assay (ELISA) was developed for determination of aculeatisides. Aculeatiside A was conjugated with bovine serum albumin (BSA) for immunization. The ratio of hapten in an antigen conjugate was determined by matrix-assisted laser desorption/ionization TOF mass spectrometry. Polyclonal antibody was developed in rabbits against an aculeatiside A-BSA conjugate. The antibody was specific for aculeatiside A and aculeatiside B. The range of the immunoassay extended from 100 ng ml-1 to 5 μg ml-1 of aculeatisides. Good correlation between ELISA and HPLC methods was obtained when crude extracts of plant samples were analyzed. The optimized ELISA was found to be applicable to the determination of total aculeatisides in various plant samples.

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Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Abstract

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