Determination and properties of acetyl conjugate of N-desisopropylpropranolol, AcNDP

Atsuko Noda, Yoko Ono, Xiuzhong Wu, Keiko Kudo, Narumi Jitsufuchi, Seiji Eto, Hiroshi Noda

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7 Citations (Scopus)


1-Acetamino-3-(1-naphthyloxy)-2-propanol (AcNDP) detected in human urine was formed as a metabolite of propranolol (PL) via 1-amino-3-(1-naphthyloxy)-2-propanol (N-desisopropylpropranolol, NDP). The excreted amount of AcNDP was determined by GC-MS using an isotope dilution method. More than 40% of total AcNDP in 24 h urine was detected 10 h after the oral administration of PL to two volunteers, and the total amounts during 24 h urine were at least 1.9–3.9% of the PL dose. As AcNDP is an intermediate metabolite of PL, its urinary amount cannot be determined exactly. Incidentally, AcNDP was chemically stable and was not formed from NDP when acetyl CoA was added to the inactivated hepatocyte system. Thus, the acetylation of NDP, an aliphatic primary amine, was confirmed to be catalyzed by N-acetyltransferase, and interestingly, the acetyl conjugation was inhibited not by sulfamethazine but by p-amino benzoic acid.

Original languageEnglish
Pages (from-to)1454-1455
Number of pages2
JournalBiological and Pharmaceutical Bulletin
Issue number10
Publication statusPublished - 1995

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science


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