TY - JOUR
T1 - Detection of the single-stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone-like protein
AU - Yokoyama, Eiji
AU - Doi, Katsumi
AU - Kimura, Makoto
AU - Ogata, Seiya
PY - 1996/5/1
Y1 - 1996/5/1
N2 - Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.
AB - Streptomyces plasmid pSA1.1 accumulated single-stranded DNA as replication intermediates in S. lividans; therefore, this plasmid was considered to replicate by a rolling-circle mechanism. A DNA-binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA-Cellulose column, then on a native DNA-Cellulose column. The N-terminal amino acid sequence of this protein has a high homology with bacterial histone-like proteins. In the gel retardation assay, this protein bound with the single-stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.
UR - http://www.scopus.com/inward/record.url?scp=0029999855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029999855&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(96)00106-1
DO - 10.1016/0378-1097(96)00106-1
M3 - Article
C2 - 9026445
AN - SCOPUS:0029999855
SN - 0378-1097
VL - 138
SP - 197
EP - 200
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 2-3
ER -