Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Yoichi Moroi, Chikage Obata, Shohei Fujita, Satoko Shibata, Sawako Konishi, Hong Duan, Shiori Saigo, Shuji Fukagawa, Akari Tashiro, Mito Toyota, Kenichi Yanagihara, Kazunori Urabe, Tetsuya Koga, Masutaka Furue

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1 Citation (Scopus)


Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalNishinihon Journal of Dermatology
Issue number2
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Dermatology


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