Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Yoichi Moroi; Chikage Obata; Shohei Fujita; Satoko Shibata; Sawako Konishi; Hong Duan; Shiori Saigo; Shuji Fukagawa; Akari Tashiro; Mito Toyota; Kenichi Yanagihara; Kazunori Urabe; Tetsuya Koga; Masutaka Furue

doi:10.2336/nishinihonhifu.64.211

Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Yoichi Moroi, Chikage Obata, Shohei Fujita, Satoko Shibata, Sawako Konishi, Hong Duan, Shiori Saigo, Shuji Fukagawa, Akari Tashiro, Mito Toyota, Kenichi Yanagihara, Kazunori Urabe, Tetsuya Koga, Masutaka Furue

Surgery

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Abstract

Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

Original language	English
Pages (from-to)	211-217
Number of pages	7
Journal	Nishinihon Journal of Dermatology
Volume	64
Issue number	2
DOIs	https://doi.org/10.2336/nishinihonhifu.64.211
Publication status	Published - 2002

All Science Journal Classification (ASJC) codes

Dermatology

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10.2336/nishinihonhifu.64.211

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title = "Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR",

abstract = "Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.",

author = "Yoichi Moroi and Chikage Obata and Shohei Fujita and Satoko Shibata and Sawako Konishi and Hong Duan and Shiori Saigo and Shuji Fukagawa and Akari Tashiro and Mito Toyota and Kenichi Yanagihara and Kazunori Urabe and Tetsuya Koga and Masutaka Furue",

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doi = "10.2336/nishinihonhifu.64.211",

language = "English",

volume = "64",

pages = "211--217",

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number = "2",

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T1 - Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

AU - Moroi, Yoichi

AU - Obata, Chikage

AU - Fujita, Shohei

AU - Shibata, Satoko

AU - Konishi, Sawako

AU - Duan, Hong

AU - Saigo, Shiori

AU - Fukagawa, Shuji

AU - Tashiro, Akari

AU - Toyota, Mito

AU - Yanagihara, Kenichi

AU - Urabe, Kazunori

AU - Koga, Tetsuya

AU - Furue, Masutaka

PY - 2002

Y1 - 2002

N2 - Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

AB - Although biopsy of sentinel lymph nodes (SLNs) has become a standard procedure for patients with melanomas, detection of micrometastases in SLNs is still difficult. To overcome this problem, we have been using RT-PCR for detection of micrometastases in SLNs. Metastatic melanoma cells in an SLN are identified with very high sensivity by RT-PCR by using primer sets of melanoma-specific antigens, such as tyrosinase, MART-1 and gp100. Histopathological re-examination is required when RT-PCR reveals positive results, because biological false-positive results are relatively frequent in RT-PCR detection of micrometastasis.

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JO - Nishinihon Journal of Dermatology

JF - Nishinihon Journal of Dermatology

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ER -

Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR

Abstract

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