Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara; Kazuhiro Iiyama; Shin Ichiro Asano; Oumi Nishi; Chisa Yasunaga-Aoki

doi:10.11416/jibs.89.2_025

Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706^t and trial for the expression of recombinant proteins in escherichia coli

Akio Kawahara, Kazuhiro Iiyama, Shin Ichiro Asano, Oumi Nishi, Chisa Yasunaga-Aoki

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706^T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.

Original language	English
Pages (from-to)	25-30
Number of pages	6
Journal	Journal of Insect Biotechnology and Sericology
Volume	89
Issue number	2
DOIs	https://doi.org/10.11416/jibs.89.2_025
Publication status	Published - 2020

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

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10.11416/jibs.89.2_025

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Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706^t and trial for the expression of recombinant proteins in escherichia coli. / Kawahara, Akio; Iiyama, Kazuhiro; Asano, Shin Ichiro et al.
In: Journal of Insect Biotechnology and Sericology, Vol. 89, No. 2, 2020, p. 25-30.

Research output: Contribution to journal › Article › peer-review

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abstract = "Two novel cry-type gene homologues, tentatively named cryPp1 and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coli was conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.",

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AU - Nishi, Oumi

AU - Yasunaga-Aoki, Chisa

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Detection of cry-type gene homologues in the draft genome sequence of paenibacillus popilliae ATCC 14706^t and trial for the expression of recombinant proteins in escherichia coli

Abstract

All Science Journal Classification (ASJC) codes

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