TY - JOUR
T1 - Detection of Candida antigen and antibody in Serum from patients with invasive candidiasis
AU - Takaki, Koji
AU - Shimono, Nobuyuki
AU - Ishimaru, Toshiyuki
AU - Okada, Kaoru
AU - Sawae, Yoshiro
AU - Niho, Yoshiyuki
PY - 1996
Y1 - 1996
N2 - Objective: To compare the sensitivity and specificity of three different assays for diagnosis of invasive candidiasis. Methods: A passive hemagglutination assay (PHA), counter-immunoelectrophoresis assay (CIE), and Cand-tec were used to test sera from 125 patients with hematologic malignancies and 65 other hospitalized patients. The former group included 15 patients with invasive candidiasis, 38 patients with Candida colonization, and 72 patients without candidiasis. Sensitivity/specificity of PHA, CIE, and Cand-tec were 87%/85%, 67%/98%, and 33%/97%, respectively. The measurement of antibody in paired sera, by PHA, was sensitive and specific; however, increased antibody titers usually occurred late in the disease. Conclusions: The combination of PHA and CIE, with a sensitivity of 67% and specificity of 98%, appeared to be the best assays for detection of invasive candidiasis in this cohort. The Cand-tec assay for Candida antigen had poor sensitivity for diagnosis of infection.
AB - Objective: To compare the sensitivity and specificity of three different assays for diagnosis of invasive candidiasis. Methods: A passive hemagglutination assay (PHA), counter-immunoelectrophoresis assay (CIE), and Cand-tec were used to test sera from 125 patients with hematologic malignancies and 65 other hospitalized patients. The former group included 15 patients with invasive candidiasis, 38 patients with Candida colonization, and 72 patients without candidiasis. Sensitivity/specificity of PHA, CIE, and Cand-tec were 87%/85%, 67%/98%, and 33%/97%, respectively. The measurement of antibody in paired sera, by PHA, was sensitive and specific; however, increased antibody titers usually occurred late in the disease. Conclusions: The combination of PHA and CIE, with a sensitivity of 67% and specificity of 98%, appeared to be the best assays for detection of invasive candidiasis in this cohort. The Cand-tec assay for Candida antigen had poor sensitivity for diagnosis of infection.
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U2 - 10.1016/S1201-9712(96)90057-0
DO - 10.1016/S1201-9712(96)90057-0
M3 - Article
AN - SCOPUS:0008562441
SN - 1201-9712
VL - 1
SP - 78
EP - 82
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
IS - 2
ER -
