Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe

Kosho Yamauchi; Yuta Matsuoka; Masatomo Takahashi; Yoshihiro Izumi; Hideto Naka; Yosuke Taniguchi; Kazuaki Kawai; Takeshi Bamba; Ken Ichi Yamada

doi:10.1039/d1cc04998d

Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe

Kosho Yamauchi, Yuta Matsuoka, Masatomo Takahashi, Yoshihiro Izumi, Hideto Naka, Yosuke Taniguchi, Kazuaki Kawai, Takeshi Bamba, Ken Ichi Yamada

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.

Original language	English
Pages (from-to)	56-59
Number of pages	4
Journal	Chemical Communications
Volume	58
Issue number	1
DOIs	https://doi.org/10.1039/d1cc04998d
Publication status	Published - Jan 4 2022

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/d1cc04998d

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title = "Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe",

abstract = "The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.",

author = "Kosho Yamauchi and Yuta Matsuoka and Masatomo Takahashi and Yoshihiro Izumi and Hideto Naka and Yosuke Taniguchi and Kazuaki Kawai and Takeshi Bamba and Yamada, {Ken Ichi}",

note = "Funding Information: This work was partially supported by the AMED-CREST grant (JP21gm0910013 to K. Y.), and the JSPS KAKENHI grants (18K14884 to Y. M.; 17H03977, 18K19405, and 20H00493 to K. Y.; 17H06304 to Y. I. and T. B.; and 17H06299 to T. B.), Japan. This work was also supported by the Platform Project for Supporting Drug Discovery and Life Science Research from AMED. We appreciate technical support provided by the Research Support Center of the Graduate School of Medical Sciences, Kyushu University. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2022",

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doi = "10.1039/d1cc04998d",

language = "English",

volume = "58",

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journal = "Chemical Communications",

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TY - JOUR

T1 - Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe

AU - Yamauchi, Kosho

AU - Matsuoka, Yuta

AU - Takahashi, Masatomo

AU - Izumi, Yoshihiro

AU - Naka, Hideto

AU - Taniguchi, Yosuke

AU - Kawai, Kazuaki

AU - Bamba, Takeshi

AU - Yamada, Ken Ichi

N1 - Funding Information: This work was partially supported by the AMED-CREST grant (JP21gm0910013 to K. Y.), and the JSPS KAKENHI grants (18K14884 to Y. M.; 17H03977, 18K19405, and 20H00493 to K. Y.; 17H06304 to Y. I. and T. B.; and 17H06299 to T. B.), Japan. This work was also supported by the Platform Project for Supporting Drug Discovery and Life Science Research from AMED. We appreciate technical support provided by the Research Support Center of the Graduate School of Medical Sciences, Kyushu University. Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2022/1/4

Y1 - 2022/1/4

N2 - The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.

AB - The oxidative damage of DNA is associated with aging and the development of various diseases. Although nucleoside-derived radicals play an important role in DNA oxidation, their analysis methods are limited. Herein, we propose a fluorometric detection and structural analysis of radicals on the surface of oxidatively damaged DNA using a profluorescent nitroxide probe combined with liquid chromatography-fluorometry and high-resolution tandem mass spectrometry.

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U2 - 10.1039/d1cc04998d

DO - 10.1039/d1cc04998d

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EP - 59

JO - Chemical Communications

JF - Chemical Communications

IS - 1

ER -

Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe

Abstract

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