TY - JOUR
T1 - Design of novel zinc finger proteins
T2 - Towards artificial control of specific gene expression
AU - Imanishi, M.
AU - Hori, Y.
AU - Nagaoka, M.
AU - Sugiura, Y.
PY - 2001
Y1 - 2001
N2 - In this review, we summarize design strategies for generating proteins with desired sequences such as long contiguous base pairs and diverse sequence specificities based on the nature of Cys2-His2 zinc finger proteins. Recent progress towards artificial DNA binding proteins has been achieved by structure-based design processes and by selection strategies. Indeed, a multi-zinc finger protein with an 18 (or 27)-base pair address, and new zinc finger proteins for diverse DNA target sites (TATA-box and p53 binding site) have been created successfully. Such novel zinc finger proteins will probably be useful tools in molecular biology and potentially in human medicine.
AB - In this review, we summarize design strategies for generating proteins with desired sequences such as long contiguous base pairs and diverse sequence specificities based on the nature of Cys2-His2 zinc finger proteins. Recent progress towards artificial DNA binding proteins has been achieved by structure-based design processes and by selection strategies. Indeed, a multi-zinc finger protein with an 18 (or 27)-base pair address, and new zinc finger proteins for diverse DNA target sites (TATA-box and p53 binding site) have been created successfully. Such novel zinc finger proteins will probably be useful tools in molecular biology and potentially in human medicine.
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U2 - 10.1016/S0928-0987(00)00212-8
DO - 10.1016/S0928-0987(00)00212-8
M3 - Review article
C2 - 11292573
AN - SCOPUS:0035061225
SN - 0928-0987
VL - 13
SP - 91
EP - 97
JO - European Journal of Pharmaceutical Sciences
JF - European Journal of Pharmaceutical Sciences
IS - 1
ER -
