Design of dual-emission chemosensors for ratiometric detection of ATP derivatives

Akio Ojida, Yoshifumi Miyahara, Jirarut Wongkongkatep, Shun Ichi Tamaru, Kazuki Sada, Itaru Hamachi

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)


Nucleoside pyrophosphate (nucleoside PP) derivatives are widespread in living cells and play pivotal roles in various biological events. We report novel fluorescence chemosensors for nucleoside PPs that make use of coordination chemistry. The chemosensors, which contain two ZnII-dipicolylamine units, bind strongly to nucleoside PPs (Kapp > 106 M-1) in aqueous solution and sense them by a dual-emission change. Detailed fluorescence and UV/Vis spectral studies revealed that the emission changes of the chemosensors upon binding to nucleoside PPs can be ascribed to the loss of coordination between ZnII and the acridine fluorophore. This is a unique sensing system based on the anion-induced rearrangement of the coordination. Furthermore, we demonstrated the utility of these chemosensors in real-time monitoring of two important biological processes involving nucleoside PP conversion: the apyrase-catalyzed hydrolysis of nucleoside PPs and the glycosyl transfer catalyzed by β-1,4-galactosyl-transferase.

Original languageEnglish
Pages (from-to)555-563
Number of pages9
JournalChemistry - An Asian Journal
Issue number4
Publication statusPublished - 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry


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