Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

Sho Hei Fujishima; Hiroshi Nonaka; Sho Hei Uchinomiya; Yoshiyuki Alex Kawase; Akio Ojida; Itaru Hamachi

doi:10.1039/c1cc16263b

Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

Sho Hei Fujishima, Hiroshi Nonaka, Sho Hei Uchinomiya, Yoshiyuki Alex Kawase, Akio Ojida, Itaru Hamachi

Molecular Bioinformatics

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A Zn(ii) complex (Zn(ii)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(ii)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.

Original language	English
Pages (from-to)	594-596
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	4
DOIs	https://doi.org/10.1039/c1cc16263b
Publication status	Published - Dec 14 2012

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "A Zn(ii) complex (Zn(ii)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(ii)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.",

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AU - Nonaka, Hiroshi

AU - Uchinomiya, Sho Hei

AU - Kawase, Yoshiyuki Alex

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2012/12/14

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AB - A Zn(ii) complex (Zn(ii)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(ii)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out.

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Design of a multinuclear Zn(ii) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins

Abstract

All Science Journal Classification (ASJC) codes

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