TY - JOUR
T1 - Dengue virus infection in aedes albopictus during the 2014 autochthonous dengue outbreak in Tokyo Metropolis, Japan
AU - Kobayashi, Daisuke
AU - Murota, Katsunori
AU - Fujita, Ryosuke
AU - Itokawa, Kentaro
AU - Kotaki, Akira
AU - Moi, Meng Ling
AU - Ejiri, Hiroko
AU - Maekawa, Yoshihide
AU - Ogawa, Kohei
AU - Tsuda, Yoshio
AU - Sasaki, Toshinori
AU - Kobayashi, Mutsuo
AU - Takasaki, Tomohiko
AU - Isawa, Haruhiko
AU - Sawabe, Kyoko
N1 - Funding Information:
We thank the Tokyo Metropolis Government, Meiji-Jingu shrine, the National Institute for Youth Education, Shinjuku Gyoen National Garden, and the Ministry of the Environment, Japan, for giving us permission to carry out the mosquito collection. We also thank the staff of the Department of Medical Entomology of National Institute of Infectious Diseases for their support to our field surveillance. This work was partly supported by grant-in-aids awarded by the Ministry of Health, Labour, and Welfare (H24-Shinko-Ippan-007), Research Program on Emerging and Reemerging Infectious Diseases (2015–2017) from Japan Agency for Medical Research and Development (AMED), and by the Japan Initiative for Global Research network on Infectious Diseases (J-GRID) (2015–2017) from Ministry of Education, Culture, Sport, Science, and Technology in Japan and AMED.
Funding Information:
Financial support: This work was partly supported by grant-in-aids awarded by the Ministry of Health, Labour, and Welfare (H24-Shinko-Ippan-007), Research Program on Emerging and Reemerging Infectious Diseases (2015–2017) from Japan Agency for Medical Research and Development (AMED), and by the Japan Initiative for Global Research network on Infectious Diseases (J-GRID) (2015–2017) from Ministry of Education, Culture, Sport, Science, and Technology in Japan and AMED.
Publisher Copyright:
Copyright © 2018 by The American Society of Tropical Medicine and Hygiene.
PY - 2018
Y1 - 2018
N2 - In 2014 in Japan, 162 autochthonous dengue cases were reported for the first time in nearly 70 years. Here, we report the results of the detection and isolation of dengue virus (DENV) from mosquitoes collected in Tokyo Metropolis in 2014 and 2015. The phylogenetic relationship among DENV isolates from mosquitoes and from patients based on both the entire envelope gene and whole coding sequences was evaluated. Herein, 2,298 female and 956 male Aedes albopictus mosquitoes were collected at six suspected locations of DENV infection in Tokyo Metropolis from August to October in 2014 and grouped into 124 and 35 pools, respectively, for viral genome detection and DENV isolation. Dengue virus RNA was detected using reverse transcription polymerase chain reaction and TaqMan assays from 49 female pools; 16 isolates were obtained using C6/36 and Vero cells. High minimum infection rates (11.2–66.7) persisted until mid-September. All DENV isolates belonged to the genotype I in serotype 1 (DENV-1), and its sequences demonstrated > 99% homology to the sequence of the DENV isolated from a patient in the vicinity of Tokyo Metropolis in 2014. Therefore, Ae. albopictus was a major DENV vector, and a single DENV-1 strain circulated in Tokyo Metropolis in 2014. Dengue virus was not detected from male mosquitoes in 2014 and wild larvae in April 2015. Thus, the possibility of both vertical transmission and overwintering of DENV was extremely low, even in dengue-epidemic areas. This study reports the first entomological information on a dengue outbreak in a temperate region, where no Aedes aegypti mosquitoes are distributed.
AB - In 2014 in Japan, 162 autochthonous dengue cases were reported for the first time in nearly 70 years. Here, we report the results of the detection and isolation of dengue virus (DENV) from mosquitoes collected in Tokyo Metropolis in 2014 and 2015. The phylogenetic relationship among DENV isolates from mosquitoes and from patients based on both the entire envelope gene and whole coding sequences was evaluated. Herein, 2,298 female and 956 male Aedes albopictus mosquitoes were collected at six suspected locations of DENV infection in Tokyo Metropolis from August to October in 2014 and grouped into 124 and 35 pools, respectively, for viral genome detection and DENV isolation. Dengue virus RNA was detected using reverse transcription polymerase chain reaction and TaqMan assays from 49 female pools; 16 isolates were obtained using C6/36 and Vero cells. High minimum infection rates (11.2–66.7) persisted until mid-September. All DENV isolates belonged to the genotype I in serotype 1 (DENV-1), and its sequences demonstrated > 99% homology to the sequence of the DENV isolated from a patient in the vicinity of Tokyo Metropolis in 2014. Therefore, Ae. albopictus was a major DENV vector, and a single DENV-1 strain circulated in Tokyo Metropolis in 2014. Dengue virus was not detected from male mosquitoes in 2014 and wild larvae in April 2015. Thus, the possibility of both vertical transmission and overwintering of DENV was extremely low, even in dengue-epidemic areas. This study reports the first entomological information on a dengue outbreak in a temperate region, where no Aedes aegypti mosquitoes are distributed.
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U2 - 10.4269/ajtmh.17-0954
DO - 10.4269/ajtmh.17-0954
M3 - Article
C2 - 29557338
AN - SCOPUS:85046906863
SN - 0002-9637
VL - 98
SP - 1460
EP - 1468
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 5
ER -
