TY - JOUR
T1 - Degradation of biodegradable plastic mulch films in soil environment by phylloplane fungi isolated from gramineous plants
AU - Koitabashi, Motoo
AU - Noguchi, Masako T.
AU - Sameshima-Yamashita, Yuka
AU - Syuntaro, Hiradate
AU - Suzuki, Ken
AU - Yoshida, Shigenobu
AU - Watanabe, Takashi
AU - Shinozaki, Yukiko
AU - Tsushima, Seiya
AU - Kitamoto, Hiroko K.
N1 - Funding Information:
We thank Showa Denko K. K. for generously supplying all the Bionolle materials (PBS and PBSA). We also acknowledge Y. T. Hoshio, S. Morimoto, and Y. Matsushita for their useful advice on PCR-DGGE technique as well as T. Fujii and E. Suto for their valuable comments on this research. This research was financially supported by the Ministry of the Environment and the National Institute for Agro-Environmental Sciences, Japan.
PY - 2012
Y1 - 2012
N2 - To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-butylene adipate) (PBSA) and poly-(butylene succinate) (PBS) films. The strain grew on the surface of soil-mounted BP films, produced breaks along the direction of hyphal growth indicated that it secreted a BP-degrading enzyme, and has directly contributing to accelerating the degradation of film. Treatment with the culture filtrate decomposed 91.2wt%, 23.7wt%, and 14.6wt% of PBSA, PBS, and commercially available BP polymer blended mulch film, respectively, on unsterlized soil within 6 days. The PCR-DGGE analysis of the transition of soil microbial community during film degradation revealed that the process was accompanied with drastic changes in the population of soil fungi and Acantamoeba spp., as well as the growth of inoculated strain B47-9. It has a potential for application in the development of an effective method for accelerating degradation of used plastics under actual field conditions
AB - To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-butylene adipate) (PBSA) and poly-(butylene succinate) (PBS) films. The strain grew on the surface of soil-mounted BP films, produced breaks along the direction of hyphal growth indicated that it secreted a BP-degrading enzyme, and has directly contributing to accelerating the degradation of film. Treatment with the culture filtrate decomposed 91.2wt%, 23.7wt%, and 14.6wt% of PBSA, PBS, and commercially available BP polymer blended mulch film, respectively, on unsterlized soil within 6 days. The PCR-DGGE analysis of the transition of soil microbial community during film degradation revealed that the process was accompanied with drastic changes in the population of soil fungi and Acantamoeba spp., as well as the growth of inoculated strain B47-9. It has a potential for application in the development of an effective method for accelerating degradation of used plastics under actual field conditions
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U2 - 10.1186/2191-0855-2-40
DO - 10.1186/2191-0855-2-40
M3 - Article
C2 - 22856640
AN - SCOPUS:84865137855
SN - 2191-0855
VL - 2
JO - AMB Express
JF - AMB Express
IS - 1
M1 - 40
ER -