Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

Hiroharu Kawahara, Koji Yamada, Kouichi Akiyama, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

Research output: Contribution to journalArticlepeer-review


To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.

Original languageEnglish
Pages (from-to)879-881
Number of pages3
JournalNippon Nogeikagaku Kaishi
Issue number5
Publication statusPublished - 1991

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemistry (miscellaneous)
  • Medicine (miscellaneous)


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