Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

Hiroharu Kawahara; Koji Yamada; Kouichi Akiyama; Hirofumi Tachibana; Sanetaka Shirahata; Hiroki Murakami

doi:10.1271/nogeikagaku1924.65.879

Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

Hiroharu Kawahara, Koji Yamada, Kouichi Akiyama, Hirofumi Tachibana, Sanetaka Shirahata, Hiroki Murakami

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.

Original language	English
Pages (from-to)	879-881
Number of pages	3
Journal	Nippon Nogeikagaku Kaishi
Volume	65
Issue number	5
DOIs	https://doi.org/10.1271/nogeikagaku1924.65.879
Publication status	Published - 1991

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Chemistry (miscellaneous)
Medicine (miscellaneous)

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10.1271/nogeikagaku1924.65.879

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title = "Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells",

abstract = "To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.",

author = "Hiroharu Kawahara and Koji Yamada and Kouichi Akiyama and Hirofumi Tachibana and Sanetaka Shirahata and Hiroki Murakami",

year = "1991",

doi = "10.1271/nogeikagaku1924.65.879",

language = "English",

volume = "65",

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journal = "Nippon Nogeikagaku Kaishi",

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T1 - Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

AU - Kawahara, Hiroharu

AU - Yamada, Koji

AU - Akiyama, Kouichi

AU - Tachibana, Hirofumi

AU - Shirahata, Sanetaka

AU - Murakami, Hiroki

PY - 1991

Y1 - 1991

N2 - To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.

AB - To analyse the amounts of various cellular components in cultured mammalian cells by flow cytometry, we tried to reduce the autologous fluorescence in human-human hybridoma HB 4 C 5 cells producing IgM. The autologous fluorescence of the cells decreased after repeated rinsing with phenol red-free MEM medium at 4°C before and after ethanol-fixation. The method could be useful in the analyses of various cellular components by flow cytometry.

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ER -

Decreasd Autologous Fluorescence in Flow Cytometry of Cultured Cells

Abstract

All Science Journal Classification (ASJC) codes

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